Development of microsatellite markers for Aquilegia buergeriana var. oxysepala (Ranunculaceae), a vulnerable Japanese herb

Tomoyuki Itagaki, Megumi K. Kimura, Chunlan Lian, Satoki Sakai

    Research output: Contribution to journalComment/debate

    2 Citations (Scopus)

    Abstract

    Aquilegia buergeriana var. oxysepala is a vulnerable herb, for which 20 microsatellite markers were developed. The applicability of these markers was confirmed by genotyping of 66 individuals collected from four natural populations in Japan. Of the 20 loci, 15 showed polymorphism. For the 15 polymorphic markers, the number of alleles per locus ranged from 2 to 12, and the expected and observed heterozygosities ranged from 0.13 to 0.77 and 0.02 to 0.65, respectively. The markers described here are sufficiently polymorphic and informative to investigate the genetic diversity and population genetic structure of A. buergeriana var. oxysepala.

    Original languageEnglish
    Pages (from-to)159-162
    Number of pages4
    JournalPlant Species Biology
    Volume30
    Issue number2
    DOIs
    Publication statusPublished - 2015 Apr 1

    Keywords

    • Aquilegia buergeriana var. oxysepala
    • Ranunculaceae
    • Simple sequence repeat
    • Vulnerable plant species

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Ecology
    • Plant Science

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