Development of mesenchymal stem cells partially originate from the neural crest

Satoru Morikawa, Yo Mabuchi, Kunimichi Niibe, Sadafumi Suzuki, Narihito Nagoshi, Takehiko Sunabori, Shigeto Shimmura, Yasuo Nagai, Taneaki Nakagawa, Hideyuki Okano, Yumi Matsuzaki

Research output: Contribution to journalArticle

174 Citations (Scopus)


Mesenchymal stem cells (MSCs) are a heterogeneous subset of stromal stem cells isolated from many adult tissues. Previous studies reported that MSCs can differentiate to both mesodermal and neural lineages by a phenomenon referred to as ''dedifferentiation'' or ''transdifferentiation''. However, since MSCs have only been defined in vitro, much of their development in vivo is still unknown. Here, we prospectively identified MSCs in the bone marrow from adult transgenic mice encoding neural crest-specific P0-Cre/Floxed-EGFP and Wnt1-Cre/Floxed-EGFP. EGFP-positive MSCs formed spheres that expressed neural crest stem cell genes and differentiated into neurons, glial cells, and myofibroblasts. Interestingly, we observed MSCs both in the GFP+ and GFP- fraction and found that there were no significant differences in the in vitro characteristics between these two populations. Our results suggest that MSCs in adult bone marrow have at least two developmental origins, one of which is the neural crest.

Original languageEnglish
Pages (from-to)1114-1119
Number of pages6
JournalBiochemical and biophysical research communications
Issue number4
Publication statusPublished - 2009 Feb 20
Externally publishedYes


  • Developmental origin
  • Flow cytometry
  • Mesenchymal stem cells
  • Neural crest
  • Prospective isolation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Morikawa, S., Mabuchi, Y., Niibe, K., Suzuki, S., Nagoshi, N., Sunabori, T., Shimmura, S., Nagai, Y., Nakagawa, T., Okano, H., & Matsuzaki, Y. (2009). Development of mesenchymal stem cells partially originate from the neural crest. Biochemical and biophysical research communications, 379(4), 1114-1119.