A novel label-free bioaffinity sensor using a lumped-constant microwave resonator was developed. A gold probe with a spherical tip immobilizing anti-chicken egg albumin antibody or anti-human albumin antibody on its surface was connected to the resonator and inserted into a flow cell to monitor the target antigen binding. The sensor showed specific sensitivity against its target antigen evidenced by the change of resonance frequency after the antigen injection. The large frequency shift observed during the injection of antigen solution was ascribed to the change of solution dielectric constant which showed a systematic increase with antigen concentration.
ASJC Scopus subject areas
- Physics and Astronomy(all)