Development of immune assay system for both CpG and non-CpG DNA from lactic acid bacteria using a transfectant of swine Toll-like receptor 9

Takeshi Shimosato, Haruki Kitazawa, Masanori Tohno, Shinichiro Katoh, Yasushi Kawai, Tadao Saito

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Bacterial DNA is expected to be a potent immune stimulating agent to Toll-like receptor (TLR) 9 expressed cells such as macrophages, monocytes, B lymphocytes, NK cells and dendritic cells. In the present study, we constructed a transfectant of swine TLR9 with mammalian cells. We demonstrated that the transfectant, recognizing both CpG and non-CpG oligonucleotides from lactic acid bacteria, induced NF-κB activation by gene reporter assay. These findings indicate that the swine TLR9 transfectant will be highly useful for the screening of immunostimulatory DNA from lactic acid bacteria.

Original languageEnglish
Pages (from-to)377-382
Number of pages6
JournalAnimal Science Journal
Volume75
Issue number4
DOIs
Publication statusPublished - 2004 Aug 1

Keywords

  • Lactic acid bacteria DNA
  • Swine
  • Toll-like receptor 9
  • Transfectant

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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