Development of human graafian follicles following transplantation of human ovarian tissue into NOD/SCID/γcnull mice

Yukihiro Terada, Yumi Terunuma-Sato, Tomoko Kakoi-yoshimoto, Hisataka Hasegawa, Tomohisa Ugajin, Yoshio Koyanagi, Mamoru Ito, Takashi Murakami, Hironobu Sasano, Nobuo Yaegashi, Kunihiro Okamura

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Problem: Transplantation of human ovarian cortex into host mice may permit various kinds of challenges in reproductive medicine. A novel immunodeficient mouse strain (NOD/SCID/γcnull: NOG) has been developed as a host of transplantation of human tissue. Method of study: Human ovarian cortex was transplanted into various sites of NOG mice and human follicular development was examined by immunohistochemistry. Results: Transplantation of human ovarian tissue into NOG mice resulted in approximately similar tissue survival and follicle growth as did transplantation into non-obese diabetic-severe combined immunodeficient mice. The human Graafian follicule from NOG mouse expressed the same steroidogenic enzymes as observed in human Graafian follicles, which developed in the human body. The NOG mice's ovarian bursa was better placed for transplantation than the back skin or kidney capsule. Conclusion: These results represent the successful generation and biological confirmation of the human Graafian follicles from the human ovarian cortex in the NOG mice.

Original languageEnglish
Pages (from-to)534-540
Number of pages7
JournalAmerican Journal of Reproductive Immunology
Volume60
Issue number6
DOIs
Publication statusPublished - 2008

Keywords

  • Graafian follicle
  • Human ovarian cortex
  • Mice
  • NOG
  • Ovarian bursa
  • Xenotransplantation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynaecology

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