Development of high density immobilized biomolecule surface for high sensitivity clinical diagnosis device

Kazuki Nishizawa, Tomohiro Konno, Madoka Takai, Kazuhiko Ishihara

Research output: Contribution to conferencePaper

Abstract

We developed a new solid biointerface by integrating a phospholipid polymer (PMBN) with a nanoscale surface modification process known as the electrospray deposition (ESD) method. In the enzyme immunoassay using this surface, the sensitivity was significantly enhanced. The surface prepared by ESD was nanosphere-shaped structure, therefore the highly uneven surface causes a drastic increase in specific signals due to an increase in the surface area. The PMBN surface which can conjugate antibodies prevented the nonspecific protein adsorption. The stability of the antibodies immobilized on the PMBN surface was improved drastically. The PMBN surface sprayed by ESD can be used to yield a highly sensitive, stable, and reliable assay.

Original languageEnglish
Pages5519-5520
Number of pages2
Publication statusPublished - 2006 Dec 1
Externally publishedYes
Event55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan
Duration: 2006 Sep 202006 Sep 22

Other

Other55th Society of Polymer Science Japan Symposium on Macromolecules
CountryJapan
CityToyama
Period06/9/2006/9/22

Keywords

  • Biointerface
  • ELISA
  • High sensitive diagnosis
  • MPC polymer

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Nishizawa, K., Konno, T., Takai, M., & Ishihara, K. (2006). Development of high density immobilized biomolecule surface for high sensitivity clinical diagnosis device. 5519-5520. Paper presented at 55th Society of Polymer Science Japan Symposium on Macromolecules, Toyama, Japan.