Development of efficient packaging method of oligodeoxynucleotides by a condensed nano particle in lipid envelope structure

Yuma Yamada, Kentaro Kogure, Yoshio Nakamura, Kaori Inoue, Hidetaka Akita, Fumi Nagatsugi, Shigeki Sasaki, Tetsuya Suhara, Hideyoshi Harashima

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

An efficient delivery system is required if antisense oligodeoxynucleotides (ODN) are to be utilized for gene therapy. We report herein on the development of a novel ODN delivery system, ODN-encapsulated nano particles (ODN-ENP) using an efficient and simple packaging method. The ODN-ENP consists of a condensed ODN particle and a lipid envelope, which can be equipped with various functional devices for the efficient delivery of ODN with a small diameter (150 nm). The encapsulation efficiency and ODN recovery of ODN-ENP were significantly higher than those of other packaging methods, such as a stabilized antisense-lipid particles method or a freeze-thaw method. Furthermore, the time required for the preparation of the ODN-ENP was shorter than the other methods. The method developed in this study is a simple and efficient packaging method for ODN with a condensed nano particle in lipid-envelope structure.

Original languageEnglish
Pages (from-to)1939-1942
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume28
Issue number10
DOIs
Publication statusPublished - 2005 Oct

Keywords

  • Condensed particle
  • Liposome
  • Nano particle
  • Oligodeoxynucleotide
  • Packaging

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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