Development of Dual Ion-selective Electrodes in Double-Barrel Glass Pipette at One Micrometer for Simultaneous Measurement of Sodium and Potassium Ions

Tomohide Takami; Yusuke Akutsu; Naoki Kaneko; Rio Yoneda; Hideyuki Magara; Shuichi Ogawa; Tadashi Abukawa

doi:10.1380/ejssnt.2023-003

Development of Dual Ion-selective Electrodes in Double-Barrel Glass Pipette at One Micrometer for Simultaneous Measurement of Sodium and Potassium Ions

Tomohide Takami, Yusuke Akutsu, Naoki Kaneko, Rio Yoneda, Hideyuki Magara, Shuichi Ogawa, Tadashi Abukawa

SR Core Research Division

Research output: Contribution to journal › Article › peer-review

Abstract

We have developed the method of preparing sodium and potassium ion-selective electrodes in double-barrel glass pipettes at the size of 1 μm. Stable and precise fabrication parameters were found for producing a double-barrel 1 μm pipette, and the produced double-barrel micropipettes were used in this study. The ionophores comprising of crown ethers of 12-crown-4 for sodium ions and 15-crown-5 for potassium ions were respectively doped in poly(vinyl chloride) films in the divided double-barrel glass pipette. The obtained selectivity coefficients and selectivity ratios are compared with the previous studies. The developed ion-selective dual-micropipettes can be applied for local ion-selective measurements such as living cells.

Original language	English
Pages (from-to)	17-23
Number of pages	7
Journal	e-Journal of Surface Science and Nanotechnology
Volume	21
Issue number	1
DOIs	https://doi.org/10.1380/ejssnt.2023-003
Publication status	Published - 2023

Keywords

Double-barrel pipette
Ion-selective electrode
Micropipette
Potassium ion
Sodium ion

ASJC Scopus subject areas

Biotechnology
Bioengineering
Condensed Matter Physics
Mechanics of Materials
Surfaces and Interfaces
Surfaces, Coatings and Films

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10.1380/ejssnt.2023-003

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title = "Development of Dual Ion-selective Electrodes in Double-Barrel Glass Pipette at One Micrometer for Simultaneous Measurement of Sodium and Potassium Ions",

abstract = "We have developed the method of preparing sodium and potassium ion-selective electrodes in double-barrel glass pipettes at the size of 1 μm. Stable and precise fabrication parameters were found for producing a double-barrel 1 μm pipette, and the produced double-barrel micropipettes were used in this study. The ionophores comprising of crown ethers of 12-crown-4 for sodium ions and 15-crown-5 for potassium ions were respectively doped in poly(vinyl chloride) films in the divided double-barrel glass pipette. The obtained selectivity coefficients and selectivity ratios are compared with the previous studies. The developed ion-selective dual-micropipettes can be applied for local ion-selective measurements such as living cells.",

keywords = "Double-barrel pipette, Ion-selective electrode, Micropipette, Potassium ion, Sodium ion",

author = "Tomohide Takami and Yusuke Akutsu and Naoki Kaneko and Rio Yoneda and Hideyuki Magara and Shuichi Ogawa and Tadashi Abukawa",

note = "Funding Information: This work was performed under the Cooperative Research Program of the Network Joint Research Center for Materials and Devices (No. 20211087), and JSPS KAKENHI Grant Number 19K05275. Publisher Copyright: {\textcopyright} 2023 The author(s)",

year = "2023",

doi = "10.1380/ejssnt.2023-003",

language = "English",

volume = "21",

pages = "17--23",

journal = "e-Journal of Surface Science and Nanotechnology",

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T1 - Development of Dual Ion-selective Electrodes in Double-Barrel Glass Pipette at One Micrometer for Simultaneous Measurement of Sodium and Potassium Ions

AU - Takami, Tomohide

AU - Akutsu, Yusuke

AU - Kaneko, Naoki

AU - Yoneda, Rio

AU - Magara, Hideyuki

AU - Ogawa, Shuichi

AU - Abukawa, Tadashi

N1 - Funding Information: This work was performed under the Cooperative Research Program of the Network Joint Research Center for Materials and Devices (No. 20211087), and JSPS KAKENHI Grant Number 19K05275. Publisher Copyright: © 2023 The author(s)

PY - 2023

Y1 - 2023

N2 - We have developed the method of preparing sodium and potassium ion-selective electrodes in double-barrel glass pipettes at the size of 1 μm. Stable and precise fabrication parameters were found for producing a double-barrel 1 μm pipette, and the produced double-barrel micropipettes were used in this study. The ionophores comprising of crown ethers of 12-crown-4 for sodium ions and 15-crown-5 for potassium ions were respectively doped in poly(vinyl chloride) films in the divided double-barrel glass pipette. The obtained selectivity coefficients and selectivity ratios are compared with the previous studies. The developed ion-selective dual-micropipettes can be applied for local ion-selective measurements such as living cells.

AB - We have developed the method of preparing sodium and potassium ion-selective electrodes in double-barrel glass pipettes at the size of 1 μm. Stable and precise fabrication parameters were found for producing a double-barrel 1 μm pipette, and the produced double-barrel micropipettes were used in this study. The ionophores comprising of crown ethers of 12-crown-4 for sodium ions and 15-crown-5 for potassium ions were respectively doped in poly(vinyl chloride) films in the divided double-barrel glass pipette. The obtained selectivity coefficients and selectivity ratios are compared with the previous studies. The developed ion-selective dual-micropipettes can be applied for local ion-selective measurements such as living cells.

KW - Double-barrel pipette

KW - Ion-selective electrode

KW - Micropipette

KW - Potassium ion

KW - Sodium ion

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JO - e-Journal of Surface Science and Nanotechnology

JF - e-Journal of Surface Science and Nanotechnology

IS - 1

ER -

Development of Dual Ion-selective Electrodes in Double-Barrel Glass Pipette at One Micrometer for Simultaneous Measurement of Sodium and Potassium Ions

Abstract

Keywords

ASJC Scopus subject areas

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