Development of dual-color simultaneous single molecule imaging system for analyzing multiple intracellular trafficking activities

Hiroyasu Hatakeyama, Makoto Kanzaki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Intracellular trafficking is a critical process for cell physiology. Previous extensive studies employing biochemical and molecular biological approaches have provided qualitative information about intracellular trafficking, but we have little quantitative information due to technical limitations of these assays. We therefore developed a novel method for quantifying intracellular trafficking based on single molecule imaging with Quantum dot (QD) fluorescent nanocrystals and quantitatively described the trafficking properties of some recycling proteins. We herein first describe how to label intracellular molecules with QD which has no cell permeability and how to quantify intracellular trafficking, and then we detail the development of a novel experimental system allowing multi-color simultaneous single molecule imaging for analyzing the relationships of intracellular trafficking activities among multiple molecules having distinct trafficking properties. Finally, we document how we confirmed the reliability of our system by simultaneously analyzing the intracellular movements of two recycling protein, GLUT4 glucose transporter and transferrin receptor. Since impairment of intracellular trafficking has critical etiological roles in various late-onset diseases such as type 2 diabetes, our novel imaging system may be a powerful tool for developing next-generation biomedical devices for diagnostics and medical treatment based on intracellular trafficking.

Original languageEnglish
Title of host publication2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
Pages1418-1421
Number of pages4
DOIs
Publication statusPublished - 2013 Oct 31
Event2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013 - Osaka, Japan
Duration: 2013 Jul 32013 Jul 7

Publication series

NameProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN (Print)1557-170X

Other

Other2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
CountryJapan
CityOsaka
Period13/7/313/7/7

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

Fingerprint Dive into the research topics of 'Development of dual-color simultaneous single molecule imaging system for analyzing multiple intracellular trafficking activities'. Together they form a unique fingerprint.

  • Cite this

    Hatakeyama, H., & Kanzaki, M. (2013). Development of dual-color simultaneous single molecule imaging system for analyzing multiple intracellular trafficking activities. In 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013 (pp. 1418-1421). [6609776] (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). https://doi.org/10.1109/EMBC.2013.6609776