Development of Arabidopsis thaliana transformants showing the self-recognition activity of Brassica rapa

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Abstract

Self-incompatibility in the Brassicaceae family is governed by two-linked highly polymorphic genes located at the S locus, SRK and SCR. Previously, the SRK and SCR genes of Arabidopsis lyrata were introduced into Arabidopsis thaliana transformants to generate self-incompatible lines. However, it has not been reported that the SRK and SCR genes of Brassica species confer self-incompatibility in A. thaliana. In this study, we attempted to construct self-incompatible A. thaliana transformants expressing the self-recognition activity of Brassica rapa by introducing the BrSCR gene along with a chimeric BrSRK gene (BrSRK chimera, in which the kinase domain of BrSRK was replaced with that of AlSKRb). We found that BrSRK chimera and BrSCR of B. rapa S-9 and S-46 haplotypes, but not those of S-29, S-44, and S-60 haplotypes, conferred self-recognition activity in A. thaliana. We also investigated the importance of amino acid residues involved in the BrSRK9–BrSCR9 interaction using A. thaliana transformants expressing mutant variants of BrSRK-9 chimera and BrSCR-9. The results showed that some of the amino acid residues are essential for self-recognition. The method developed in this study for the construction of self-incompatible A. thaliana transformants showing B. rapa self-recognition activity will be useful for analysis of self-recognition mechanisms in Brassicaceae.

Original languageEnglish
JournalUnknown Journal
DOIs
Publication statusPublished - 2020 Jul 16

Keywords

  • Arabidopsis
  • Brassica
  • Brassicaceae
  • Receptor kinase
  • SCR
  • Self-incompatibility
  • SRK

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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