For the determination of stroma content in SFH, two analytical methods were developed, the HPLC-UV for phospholipids and the enzyme immunoassay for blood group antigen. These analytical methods were applied to evaluate three representative SFH preparations. The 36,000 x g centrifugation method was shown to contain higher amount of stroma components. On the other hand, the BMM-40nm and the ultrafiltration method with a 100kd membrane was shown to remove more than 99.7% of phospholipids and almost all of blood group antigen from hemoglobin solutions. These results demonstrated that these analytical methods were very useful in evaluating any highly purified hemoglobin solutions.
|Number of pages||5|
|Journal||Biomaterials, Artificial Cells and Immobilization Biotechnology|
|Issue number||4 --Feb|
|Publication status||Published - 1992 Jan|
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