Development of analytical methods to evaluate SFH

K. Nakai, H. Abe, N. Matsuda, M. Kobayashi, H. Ikeda, S. Sekiguchi, E. Tsuchida

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


For the determination of stroma content in SFH, two analytical methods were developed, the HPLC-UV for phospholipids and the enzyme immunoassay for blood group antigen. These analytical methods were applied to evaluate three representative SFH preparations. The 36,000 x g centrifugation method was shown to contain higher amount of stroma components. On the other hand, the BMM-40nm and the ultrafiltration method with a 100kd membrane was shown to remove more than 99.7% of phospholipids and almost all of blood group antigen from hemoglobin solutions. These results demonstrated that these analytical methods were very useful in evaluating any highly purified hemoglobin solutions.

Original languageEnglish
Pages (from-to)447-451
Number of pages5
JournalBiomaterials, Artificial Cells and Immobilization Biotechnology
Issue number4 --Feb
Publication statusPublished - 1992 Jan
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


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