TY - JOUR
T1 - Development of analytical methods to evaluate SFH
AU - Nakai, K.
AU - Abe, H.
AU - Matsuda, N.
AU - Kobayashi, M.
AU - Ikeda, H.
AU - Sekiguchi, S.
AU - Tsuchida, E.
PY - 1992/1
Y1 - 1992/1
N2 - For the determination of stroma content in SFH, two analytical methods were developed, the HPLC-UV for phospholipids and the enzyme immunoassay for blood group antigen. These analytical methods were applied to evaluate three representative SFH preparations. The 36,000 x g centrifugation method was shown to contain higher amount of stroma components. On the other hand, the BMM-40nm and the ultrafiltration method with a 100kd membrane was shown to remove more than 99.7% of phospholipids and almost all of blood group antigen from hemoglobin solutions. These results demonstrated that these analytical methods were very useful in evaluating any highly purified hemoglobin solutions.
AB - For the determination of stroma content in SFH, two analytical methods were developed, the HPLC-UV for phospholipids and the enzyme immunoassay for blood group antigen. These analytical methods were applied to evaluate three representative SFH preparations. The 36,000 x g centrifugation method was shown to contain higher amount of stroma components. On the other hand, the BMM-40nm and the ultrafiltration method with a 100kd membrane was shown to remove more than 99.7% of phospholipids and almost all of blood group antigen from hemoglobin solutions. These results demonstrated that these analytical methods were very useful in evaluating any highly purified hemoglobin solutions.
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U2 - 10.3109/10731199209119667
DO - 10.3109/10731199209119667
M3 - Article
C2 - 1391462
AN - SCOPUS:0026728054
VL - 20
SP - 447
EP - 451
JO - Artificial Cells, Nanomedicine and Biotechnology
JF - Artificial Cells, Nanomedicine and Biotechnology
SN - 2169-1401
IS - 4 --Feb
ER -