Development of analytical methods to evaluate SFH

K. Nakai, H. Abe, N. Matsuda, M. Kobayashi, H. Ikeda, S. Sekiguchi, E. Tsuchida

Research output: Contribution to journalConference articlepeer-review

Abstract

It is known that red cell stroma stimulate blood coagulation, which may result in several toxic effects including DIC and renal failure. This coagulation activity of the stroma was examined in vitro. Anti-phosphatidylserine (PS) antibody inhibited the coagulation activity of the stroma. While the coagulation activity of the hemolysate was different in each hemolyzing method, freezing and thawing of the preparations uniformally produced an increase in coagulation activity irrespective of the hemolyzing method. Since most PS is known to be present in the inner leaflet of the cell membrane and PS increases coagulation activity, these results suggest that the increased coagulation activity of the hemolysate is due to the externalization of PS onto the outer leaflet of the stroma during hemolysis or during freezing and thawing, and that it is important to measure the PS content in SFH for quality control. A HPLC-UV system was developed to measure each phospholipid class. This method has proved to be more specific and sensitive than the phosphorus assay. The ELISA system for blood group antigen was also constructed as an additional monitoring method for residual stroma. These methods were useful in evaluating a highly purified SFH. The above assays were utilized to compare SFH solutions prepared by several methods, and to establish our preparation method, which would produce a highly qualified SFH in combination with a virus-removal filter, BMM.

Original languageEnglish
Number of pages1
JournalBiomaterials, Artificial Cells, and Immobilization Biotechnology
Volume19
Issue number2
Publication statusPublished - 1991 Dec 1
Externally publishedYes
Event8th World Congress of the International Society for Artificial Organs in conjunction with the 4th International Symposium on Blood Substitutes - Montreal, Que, Can
Duration: 1991 Aug 191991 Aug 23

ASJC Scopus subject areas

  • Medicine(all)

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