Development of an ultrasound microscope combined with optical microscope for multiparametric characterization of a single cell

Mototaka Arakawa, Joe Shikama, Koki Yoshida, Ryo Nagaoka, Kazuto Kobayashi, Yoshifumi Saijo

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Biomechanics of the cell has been gathering much attention because it affects the pathological status in atherosclerosis and cancer. In the present study, an ultrasound microscope system combined with optical microscope for characterization of a single cell with multiple ultrasound parameters was developed. The central frequency of the transducer was 375 MHz and the scan area was 80 x 80 μm with up to 200 x 200 sampling points. An inverted optical microscope was incorporated in the design of the system, allowing for simultaneous optical observations of cultured cells. Two-dimensional mapping of multiple ultrasound parameters, such as sound speed, attenuation, and acoustic impedance, as well as the thickness, density, and bulk modulus of specimen/cell under investigation, etc., was realized by the system. Sound speed and thickness of a 3T3-L1 fibroblast cell were successfully obtained by the system. The ultrasound microscope system combined with optical microscope further enhances our understanding of cellular biomechanics.

Original languageEnglish
Article number7272460
Pages (from-to)1615-1622
Number of pages8
JournalIEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
Volume62
Issue number9
DOIs
Publication statusPublished - 2015 Sep 1

Keywords

  • Acoustics
  • Biomedical optical imaging
  • Microscopy
  • Optical attenuators
  • Optical imaging
  • Optical microscopy
  • Ultrasonic imaging

ASJC Scopus subject areas

  • Instrumentation
  • Acoustics and Ultrasonics
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Development of an ultrasound microscope combined with optical microscope for multiparametric characterization of a single cell'. Together they form a unique fingerprint.

Cite this