Development of an in vitro photosensitization test based on changes of cell-surface thiols and amines as biomarkers: The photo-SH/NH2 test

Shiho Oeda, Morihiko Hirota, Hayato Nishida, Takao Ashikaga, Hitoshi Sasa, Setsuya Aiba, Yoshiki Tokura, Hirokazu Kouzuki

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

As a part of our studies to develop a cell-based in vitro photosensitization assay, we examined whether changes of cell-surface thiols and amines on human monocytic cell line THP-1 could be used to predict photosensitizing potential of chemicals. First, we identified a suitable ultraviolet A (UV-A) irradiation dose to be 5.0 J/cm2 by investigating the effect of UV-A on the levels of cell-surface thiols and amines in ketoprofen (KP; a representative photoallergen)-treated THP-1 cells. Next, we confirmed that phenol red, a known photoirritant used as a pH indicator in the culture medium, did not affect the KP-induced changes of cell-surface thiols and amines. Using the criterion of more than 15% change of cell-surface thiols and/or amines in response to UV-A irradiation, 22 of 26 known photosensitizers (15 of 18 photoallergens, 7 of 8 photoirritants) were judged positive. Seven of 7 known non-phototoxins did not alter cell-surface thiols or amines. The accuracy for predicting photosensitizers was 87.9% (sensitivity/specificity; 84.6%/100%), and the accuracy for predicting photoallergens was 69.7% (sensitivity/specificity; 83.3%/53.3%). Our results suggest that changes of cell-surface thiols and/or amines may be useful biomarkers for predicting photosensitization potential, including photoallergenicity, of compounds. We designate this test as the photo-SH/NH2 test.

Original languageEnglish
Pages (from-to)129-142
Number of pages14
JournalJournal of Toxicological Sciences
Volume41
Issue number1
DOIs
Publication statusPublished - 2016 Jan 13

Keywords

  • Cell-surface thiols and amines
  • In vitro
  • Photo-SH/NH test
  • Photosensitization
  • THP-1 cells

ASJC Scopus subject areas

  • Toxicology

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