Development of an experimental model of infected skin ulcer

Masahiro Tachi, Shinichi Hirabayashi, Yoshiyuki Yonehara, Yasutoshi Suzuki, Philip Bowler

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A model of infected skin ulceration could prove useful in assessing the clinical effectiveness of antimicrobial ointments and dressings. However, no such models have been previously established. Three types of wound were induced in rats: full-thickness wounds covered with gauze, burn wounds and wounds resulting from mechanical trauma. Wounds were inoculated with S. aureus or P. aeruginosa. Persistent infected wounds were observed only in full-thickness wounds covered with gauze. In a second experiment, colonies of P. aeruginosa or S. aureus were counted within 15×15mm full-thickness wounds covered with gauze. Wounds were inoculated with 1 0 × 106 colony-forming units (CFU) of P. aeruginosa or S. aureus and then sealed to ensure an enclosed environment. Tissue bacterial counts exceeded 106 CFU/g from the next day until day 9 after infection. Bacterial counts exceeded 108 CFU/ml in wound exudate collected between days 1 and 7. We have developed a model of wound infection in which persistence of infection can be achieved for 9 days following ulceration due to the application of gauze to the base of a full-thickness wound.

Original languageEnglish
Pages (from-to)49-55
Number of pages7
JournalInternational wound journal
Volume1
Issue number1
DOIs
Publication statusPublished - 2004

Keywords

  • Animal model
  • Colonisation
  • Rat
  • S. Aureus
  • Wound infection

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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