Development of an Engineered Photoactive Yellow Protein as a Cross-Linking Junction for Construction of Photoresponsive Protein-Polymer Conjugates

Mihoko Ui, Yusuke Miyauchi, Masataka Inoue, Makoto Murakami, Yasuyuki Araki, Takehiko Wada, Kazushi Kinbara

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Based on a bacterial blue light photoreceptor, photoactive yellow protein (PYP), we have developed a photoresponsive protein module, Hpg5-PYP, bearing five alkynyl groups as reactive sites. We constructed a yellow-colored photoresponsive protein–polymer conjugate by using covalently bonded Hpg5-PYP with poly(N,N-dimethylacrylamide-co-2-hydroxy-3-azidopropyl methacrylate) (DMA-HAZPMA copolymer). Hpg5-PYP retained photoreactivity towards blue-light irradiation in the conjugate, and the conjugate showed a photoresponsive viscosity change upon irradiation with blue light at 25 °C. In contrast, a mixture of Hpg5-PYP and DMA-HAZPMA copolymer prepared for reference did not show any dependency of the viscosity on light irradiation, indicating the importance of crosslinking.

Original languageEnglish
Pages (from-to)356-360
Number of pages5
JournalChemPhotoChem
Volume3
Issue number6
DOIs
Publication statusPublished - 2019 Jun 1

Keywords

  • bioconjugate materials
  • photoactive yellow protein
  • photoreceptors
  • photoresponsive materials
  • protein-polymer conjugates
  • viscosity

ASJC Scopus subject areas

  • Analytical Chemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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