Development of an automated large-scale protein-crystallization and monitoring system for high-throughput protein-structure analyses

Masahiko Hiraki, Ryuichi Kato, Minoru Nagai, Tadashi Satoh, Satoshi Hirano, Kentaro Ihara, Norio Kudo, Masamichi Nagae, Masanori Kobayashi, Michio Inoue, Tamami Uejima, Shunichiro Oda, Leonard M.G. Chavas, Masato Akutsu, Yusuke Yamada, Masato Kawasaki, Naohiro Matsugaki, Noriyuki Igarashi, Mamoru Suzuki, Soichi Wakatsuki

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

Protein crystallization remains one of the bottlenecks in crystallographic analysis of macromolecules. An automated large-scale protein-crystallization system named PXS has been developed consisting of the following subsystems, which proceed in parallel under unified control software: dispensing precipitants and protein solutions, sealing crystallization plates, carrying robot, incubators, observation system and image-storage server. A sitting-drop crystallization plate specialized for PXS has also been designed and developed. PXS can set up 7680 drops for vapour diffusion per hour, which includes time for replenishing supplies such as disposable tips and crystallization plates. Images of the crystallization drops are automatically recorded according to a preprogrammed schedule and can be viewed by users remotely using web-based browser software. A number of protein crystals were successfully produced and several protein structures could be determined directly from crystals grown by PXS. In other cases, X-ray quality crystals were obtained by further optimization by manual screening based on the conditions found by PXS.

Original languageEnglish
Pages (from-to)1058-1065
Number of pages8
JournalActa Crystallographica Section D: Biological Crystallography
Volume62
Issue number9
DOIs
Publication statusPublished - 2006 Sep

ASJC Scopus subject areas

  • Structural Biology

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