Development of an Aspergillus oryzae whole-cell biocatalyst coexpressing triglyceride and partial glyceride lipases for biodiesel production

Daisuke Adachi, Shinji Hama, Takao Numata, Kazunori Nakashima, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

An Aspergillus oryzae whole-cell biocatalyst which coexpresses Fusarium heterosporum lipase (FHL) and mono- and di-acylglycerol lipase B (mdlB) in the same cell has been developed to improve biodiesel production. By screening a number of transformants, the best strain was obtained when FHL gene was integrated into A. oryzae chromosome using sC selection marker while mdlB was integrated using niaD selection marker. The reaction system using the lipase-coexpressing whole-cells was found to be superior in biodiesel production to others such as lipase-mixing and two-step reactions, affording the highest reaction rate and the highest ME content (98%). Moreover, an ME content of more than 90% was maintained during 10 repeated batch cycles. The whole-cell biocatalyst developed in this work would be promising biocatalysts for efficient biodiesel production.

Original languageEnglish
Pages (from-to)6723-6729
Number of pages7
JournalBioresource Technology
Volume102
Issue number12
DOIs
Publication statusPublished - 2011 Jun 1

Keywords

  • Biodiesel fuel
  • Biomass support particles
  • Coexpression
  • Lipase
  • Whole-cell biocatalyst

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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