Development of an anti-bear podoplanin monoclonal antibody PMab-247 for immunohistochemical analysis

Yoshikazu Furusawa, Junko Takei, Yusuke Sayama, Shinji Yamada, Mika K. Kaneko, Yukinari Kato

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Sensitive and specific monoclonal antibodies (mAbs)targeting podoplanin (PDPN)are needed for immunohistochemical analyses using formalin-fixed paraffin-embedded tissues because PDPN is known as a lymphatic endothelial cell maker in pathology. Recently, we established anti-PDPN mAbs against many species, such as human, mouse, rat, rabbit, dog, cat, bovine, pig, horse, goat, tiger, alpaca, and Tasmanian devil. However, anti-bear PDPN (bPDPN)has not been established yet. In this study, we immunized mice with bPDPN-overexpressing Chinese hamster ovary (CHO)-K1 (CHO/bPDPN)cells, and screened mAbs against bPDPN using flow cytometry. One of the mAbs, PMab-247 (IgG1, kappa), specifically detected CHO/bPDPN cells by flow cytometry and immunohistochemistry. Our findings suggest the potential usefulness of PMab-247 for the functional analyses of bPDPN.

Original languageEnglish
Article number100644
JournalBiochemistry and Biophysics Reports
Volume18
DOIs
Publication statusPublished - 2019 Jul

Keywords

  • Bear podoplanin
  • Immunohistochemistry
  • PDPN
  • PMab-247

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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