Development of an amperometric l-lactate biosensor based on l-lactate oxidase immobilized through silica sol-gel film on multi-walled carbon nanotubes/platinum nanoparticle modified glassy carbon electrode

Jiadong Huang, Jing Li, Yu Yang, Xinsheng Wang, Baoyan Wu, Jun ichi Anzai, Tetsuo Osa, Qiang Chen

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Platinum nanoparticles (Ptnano) were used in combination with multi-walled carbon nanotubes (MWCNTs) for fabricating sensitivity-enhanced electrochemical l-lactate biosensor. The composite film of MWCNTs and Ptnano was dispersed on the surface of the glassy carbon electrode (GCE). l-lactate oxidase (LOD) was immobilized on MWCNTs/Ptnano/GCE surface by adsorption. The resulting LOD/MWCNTs/Ptnano electrode was covered by a thin layer of sol-gel to avoid the loss of LOD in determination and to improve the anti-interferent ability. Moreover, the sol-gel microenviroment contributes to both intensified stability and permselectivity. The cyclic voltammetry results indicated that MWCNTs/Ptnano catalyst displayed a higher performance than MWCNTs. Under the optimized conditions of applied potential 0.5 V, pH 6.4, room temperature, the proposed biosensor showed a large determination range (0.2-2.0 mM), a short response time (within 5 s), a high sensitivity (6.36 μA mM- 1) and good stability (90% remains after 4 weeks). The fabricated biosensor had practically good selectivity against interferences. The results for whole blood samples measured by the present biosensor showed a good agreement with those measured by spectrophotometric method.

Original languageEnglish
Pages (from-to)1070-1075
Number of pages6
JournalMaterials Science and Engineering C
Volume28
Issue number7
DOIs
Publication statusPublished - 2008 Aug 1

Keywords

  • Biosensor
  • Multi-walled carbon nanotubes
  • Platinum nanoparticle
  • Sol-gel
  • l-lactate oxidase

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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