TY - JOUR
T1 - Development of a spreadsheet for SNPs typing using MicrosoftR EXCEL
AU - Hashiyada, Masaki
AU - Itakura, Yukio
AU - Takahashi, Shirushi
AU - Sakai, Jun
AU - Funayama, Masato
N1 - Funding Information:
This work was supported in parts by The Ministry of Education, Culture, Sports, Science and Technology (MEXT) Grant-in-Aid for Scientific Research on Priority Areas titled “New IT Infrastructure for the Information-explosion Era”.
PY - 2009/4
Y1 - 2009/4
N2 - Single-nucleotide polymorphisms (SNPs) have some characteristics that make them very appropriate for forensic studies and applications. In our institute, SNPs typings were performed by the TaqManR SNP Genotyping Assays using the ABI PRISM 7500 FAST Real-Time PCR System (AppliedBiosystems) and Sequence Detection Software ver.1.4 (AppliedBiosystem). The TaqMan method was desired two positive control (Allele1 and 2) and one negative control to analyze each SNP locus. Therefore, it can be analyzed up to 24 loci of a person on a 96-well-plate at the same time. If SNPs analysis is expected to apply to biometrics authentication, 48 and over loci are required to identify a person. In this study, we designed a spreadsheet package using MicrosoftR EXCEL, and population data were used from our 120 SNPs population studies. On the spreadsheet, we defined SNP types using 'template files' instead of positive and negative controls. "Template files" consisted of the results of 94 unknown samples and two negative controls of each of 120 SNPs loci we had previously studied. By the use of the files, the spreadsheet could analyze 96 SNPs on a 96-wells-plate simultaneously.
AB - Single-nucleotide polymorphisms (SNPs) have some characteristics that make them very appropriate for forensic studies and applications. In our institute, SNPs typings were performed by the TaqManR SNP Genotyping Assays using the ABI PRISM 7500 FAST Real-Time PCR System (AppliedBiosystems) and Sequence Detection Software ver.1.4 (AppliedBiosystem). The TaqMan method was desired two positive control (Allele1 and 2) and one negative control to analyze each SNP locus. Therefore, it can be analyzed up to 24 loci of a person on a 96-well-plate at the same time. If SNPs analysis is expected to apply to biometrics authentication, 48 and over loci are required to identify a person. In this study, we designed a spreadsheet package using MicrosoftR EXCEL, and population data were used from our 120 SNPs population studies. On the spreadsheet, we defined SNP types using 'template files' instead of positive and negative controls. "Template files" consisted of the results of 94 unknown samples and two negative controls of each of 120 SNPs loci we had previously studied. By the use of the files, the spreadsheet could analyze 96 SNPs on a 96-wells-plate simultaneously.
KW - Matching probability
KW - Microsoft EXCEL
KW - SNP
KW - TaqMan
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U2 - 10.1016/j.legalmed.2009.01.047
DO - 10.1016/j.legalmed.2009.01.047
M3 - Article
C2 - 19261525
AN - SCOPUS:67349174537
VL - 11
SP - S453-S454
JO - Legal Medicine
JF - Legal Medicine
SN - 1344-6223
IS - SUPPL. 1
ER -