Development of a spreadsheet for SNPs typing using MicrosoftR EXCEL

Masaki Hashiyada, Yukio Itakura, Shirushi Takahashi, Jun Sakai, Masato Funayama

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Single-nucleotide polymorphisms (SNPs) have some characteristics that make them very appropriate for forensic studies and applications. In our institute, SNPs typings were performed by the TaqManR SNP Genotyping Assays using the ABI PRISM 7500 FAST Real-Time PCR System (AppliedBiosystems) and Sequence Detection Software ver.1.4 (AppliedBiosystem). The TaqMan method was desired two positive control (Allele1 and 2) and one negative control to analyze each SNP locus. Therefore, it can be analyzed up to 24 loci of a person on a 96-well-plate at the same time. If SNPs analysis is expected to apply to biometrics authentication, 48 and over loci are required to identify a person. In this study, we designed a spreadsheet package using MicrosoftR EXCEL, and population data were used from our 120 SNPs population studies. On the spreadsheet, we defined SNP types using 'template files' instead of positive and negative controls. "Template files" consisted of the results of 94 unknown samples and two negative controls of each of 120 SNPs loci we had previously studied. By the use of the files, the spreadsheet could analyze 96 SNPs on a 96-wells-plate simultaneously.

Original languageEnglish
Pages (from-to)S453-S454
JournalLegal Medicine
Volume11
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 2009 Apr

Keywords

  • Matching probability
  • Microsoft EXCEL
  • SNP
  • TaqMan

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Issues, ethics and legal aspects

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