A quantitative method for collection of periapical exudates on endodontic paper points is described. In a pilot study, it was revealed that the relationship between fluid volume and wetted length of paper points had a highly significant curvilinear relationship (p < 0.0001), and a highly significant positive linear relationship was found to describe eluted and absorbed interleukin (IL)-1β activities from paper points. Periapical exudates from 29 root canals with apical periodontitis were collected using this method, and IL-1β activities in clinical specimens were measured. Periapical exudates (0.15 to 26.7 μl) were recovered, and the range of IL-1β concentration was 0.1 to 179.5 ng/ml. These results showed that this sampling method was useful to analyze immunological changes in periapical lesions.
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