Development of a quantitative sampling method for periapical exudates from human root canals

Hidetoshi Shimauchi; Yasuo Miki; Shin Ichi Takayama; Takako Imai; Hiroshi Okada

doi:10.1016/S0099-2399(96)80032-X

Development of a quantitative sampling method for periapical exudates from human root canals

Hidetoshi Shimauchi, Yasuo Miki, Shin Ichi Takayama, Takako Imai, Hiroshi Okada

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

A quantitative method for collection of periapical exudates on endodontic paper points is described. In a pilot study, it was revealed that the relationship between fluid volume and wetted length of paper points had a highly significant curvilinear relationship (p < 0.0001), and a highly significant positive linear relationship was found to describe eluted and absorbed interleukin (IL)-1β activities from paper points. Periapical exudates from 29 root canals with apical periodontitis were collected using this method, and IL-1β activities in clinical specimens were measured. Periapical exudates (0.15 to 26.7 μl) were recovered, and the range of IL-1β concentration was 0.1 to 179.5 ng/ml. These results showed that this sampling method was useful to analyze immunological changes in periapical lesions.

Original language	English
Pages (from-to)	612-615
Number of pages	4
Journal	Journal of endodontics
Volume	22
Issue number	11
DOIs	https://doi.org/10.1016/S0099-2399(96)80032-X
Publication status	Published - 1996
Externally published	Yes

ASJC Scopus subject areas

Dentistry(all)

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10.1016/S0099-2399(96)80032-X

Cite this

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title = "Development of a quantitative sampling method for periapical exudates from human root canals",

abstract = "A quantitative method for collection of periapical exudates on endodontic paper points is described. In a pilot study, it was revealed that the relationship between fluid volume and wetted length of paper points had a highly significant curvilinear relationship (p < 0.0001), and a highly significant positive linear relationship was found to describe eluted and absorbed interleukin (IL)-1β activities from paper points. Periapical exudates from 29 root canals with apical periodontitis were collected using this method, and IL-1β activities in clinical specimens were measured. Periapical exudates (0.15 to 26.7 μl) were recovered, and the range of IL-1β concentration was 0.1 to 179.5 ng/ml. These results showed that this sampling method was useful to analyze immunological changes in periapical lesions.",

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T1 - Development of a quantitative sampling method for periapical exudates from human root canals

AU - Shimauchi, Hidetoshi

AU - Miki, Yasuo

AU - Takayama, Shin Ichi

AU - Imai, Takako

AU - Okada, Hiroshi

PY - 1996

Y1 - 1996

N2 - A quantitative method for collection of periapical exudates on endodontic paper points is described. In a pilot study, it was revealed that the relationship between fluid volume and wetted length of paper points had a highly significant curvilinear relationship (p < 0.0001), and a highly significant positive linear relationship was found to describe eluted and absorbed interleukin (IL)-1β activities from paper points. Periapical exudates from 29 root canals with apical periodontitis were collected using this method, and IL-1β activities in clinical specimens were measured. Periapical exudates (0.15 to 26.7 μl) were recovered, and the range of IL-1β concentration was 0.1 to 179.5 ng/ml. These results showed that this sampling method was useful to analyze immunological changes in periapical lesions.

AB - A quantitative method for collection of periapical exudates on endodontic paper points is described. In a pilot study, it was revealed that the relationship between fluid volume and wetted length of paper points had a highly significant curvilinear relationship (p < 0.0001), and a highly significant positive linear relationship was found to describe eluted and absorbed interleukin (IL)-1β activities from paper points. Periapical exudates from 29 root canals with apical periodontitis were collected using this method, and IL-1β activities in clinical specimens were measured. Periapical exudates (0.15 to 26.7 μl) were recovered, and the range of IL-1β concentration was 0.1 to 179.5 ng/ml. These results showed that this sampling method was useful to analyze immunological changes in periapical lesions.

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Development of a quantitative sampling method for periapical exudates from human root canals

Abstract

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