Development of a micro-imaging probe for functional brain imaging

Makoto Osanai, Taro Suzuki, Atsushi Tamura, Tsugio Yonemura, Issei Mori, Yuchio Yanagawa, Hiromu Yawo, Hajime Mushiake

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Multicellular neuronal activities should be investigated to reveal the dynamics of the neuronal circuit. Optical recording from neuronal populations is suitable for recording multicellular activities. We fabricated the prototype of the micro-imaging probe in combination with a gradient index lens and image fiber. This probe has a smaller diameter than traditional probes. We found an optimal optical configuration for maximizing the efficiency of the imaging probe. Using this optical configuration with the prototype of the imaging probe, the fluorescence images were captured from neurons expressing green fluorescent protein in a cerebellar block preparation, and the calcium-dependent images were sampled in a mouse brain slice preparation. Our optical system would facilitate the in vivo imaging studies with less invasive manners using thinner optic fiber than previously made.

Original languageEnglish
Pages (from-to)46-52
Number of pages7
JournalNeuroscience Research
Volume75
Issue number1
DOIs
Publication statusPublished - 2013 Jan 1

Keywords

  • Endoscope
  • GRIN lens
  • Image fiber
  • Imaging
  • Neuronal circuit

ASJC Scopus subject areas

  • Neuroscience(all)

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  • Cite this

    Osanai, M., Suzuki, T., Tamura, A., Yonemura, T., Mori, I., Yanagawa, Y., Yawo, H., & Mushiake, H. (2013). Development of a micro-imaging probe for functional brain imaging. Neuroscience Research, 75(1), 46-52. https://doi.org/10.1016/j.neures.2012.10.008