Development of a method to detect viral RNA sequences from cultured cells by combining size fraction and a rapid determination system for viral RNA sequences (RDV)

Shumpei Watanabe, Tetsuya Mizutani, Kouji Sakai, Itoe Iizuka, Tomoyuki Shiota, Yusuke Sayama, Shumpei Tsuda, Kentaro Kato, Shuetsu Fukushi, Masayuki Saijo, Ichiro Kurane, Shigeru Morikawa, Hiroomi Akashi

Research output: Contribution to journalArticle

Abstract

By combining size-fraction and a rapid determination system for viral RNA sequences (RDV), a method for sequence independent detection from virus-infected cultured cells was developed as "RDV-size fraction (RDV-SF)". Using RDV-SF, we succeeded in detecting nucleotide fragment sequences of feline calicivirus (FCV) and Yokose virus (YOKV) from Vero E6 cells infected with FCV and YOKV, respectively. RDV-SF was useful in identifying RNA viruses with a genome size of more than 5 kb from virus-infected cultured cells.

Original languageEnglish
JournalJournal of Veterinary Science and Technology
Volume1
Issue number1
DOIs
Publication statusPublished - 2012 Dec 1
Externally publishedYes

Keywords

  • Direct sequencing
  • RDV-SF
  • RNA virus
  • Sequence-independent detection

ASJC Scopus subject areas

  • veterinary(all)

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