Development of a fluorescent indicator for the bioimaging of nitric oxide

Hirotatsu Kojima, Kuniko Sakurai, Kazuya Kikuchi, Shigenori Kawahara, Yutaka Kirino, Hiroshi Nagoshi, Yasunobu Hirata, Takaaki Akaike, Hiroshi Maeda, Tetsuo Nagano

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

Nitric oxide (NO) has been reported to play various roles as a signal transmitter. However, detailed functions of NO have yet to be clarified. We have developed a fluorescent indicator for NO imaging in living cells. The N- nitrosation of newly designed and synthesized 4-((3-amino-2- naphthyl)aminomethyl)benzoic acid (DAN-1) by NO yielded the highly fluorescent triazole-form. The membrane permeable ester derivative of DAN-1 (DAN-1 EE) was applied to the imaging of NO produced in activated rat aortic smooth muscle cells. After DAN-1 EE has been loaded into cells, the ester bond is hydrolyzed by intracellular esterase, yielding original DAN-1 with less permeability. The fluorescence intensity of the cells loaded with DAN-1 EE increased according to NO production. The imaging method with fluorescent indicators will be significant for the functional clarification of NO in vivo.

Original languageEnglish
Pages (from-to)1229-1232
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume20
Issue number12
DOIs
Publication statusPublished - 1997

Keywords

  • Diaminonaphthalene
  • Fluorescent indicator
  • Imaging
  • Nitric oxide
  • Vascular smooth muscle cell

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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