Development of a crystallization protocol for the DbeAl variant of novel haloalkane dehalogenase from bradyrhizobium elkani USDA94

T. Prudnikova, R. Chaloupkova, Y. Sato, Y. Nagata, O. Degtjarik, M. Kuty, P. Rezacova, J. Damborsky, J. Kuta Smatanova

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The DbeA1 variant of a novel haloalkane dehalogenase DbeA (EC 3.8.1.5) from Bradyrhizobium elkani USDA94 was constructed to study the structure-function relationships between DbjA and DbeA enzymes. A DbeA1 variant carries a unique fragment of nine amino acids transplanted from the sequentially closely related enzyme DbjA from Bradyrhizobium japonicum USDA110 to DbeA. Here we report development of the crystallization protocol for DbeA1 and soaking experiments with the ligands 1-fluoropentane and 1, 3-dichloropropane.

Original languageEnglish
Pages (from-to)516-519
Number of pages4
JournalCrystal Growth and Design
Volume11
Issue number2
DOIs
Publication statusPublished - 2011 Feb 2

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

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