Development and validation of a microarray for the confirmation and typing of norovirus RT-PCR products

Kirsten Mattison, Nathalie Corneau, Ingvild Berg, Albert Bosch, Erwin Duizer, Ion Gutiérrez-Aguirre, Yvan L'Homme, Yalda Lucero, Zhiyao Luo, Alexander Martyres, Mette Myrmel, Miguel O'Ryan, Franco Pagotto, Daisuke Sano, Sanela Svraka, Ulises Urzua, Sabah Bidawid

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Noroviruses are implicated in many worldwide institutional, food and waterborne outbreaks each year. Genetic typing of isolates is valuable for monitoring outbreak spread as well as variation in circulating strains. Microarrays have the potential to provide rapid genotype information for norovirus samples. The NoroChip v3.0 provides an oligonucleotide hybridization platform to screen for over 600 potential interactions in each experiment. The NoroChip v3.0 was developed at Health Canada and validated in seven international partner laboratories. Each laboratory validated the NoroChip v3.0 using norovirus amplicons routinely characterized in their testing protocols. Fragments from the capsid region (region C) and a 2.4. kb amplicon spanning polymerase and capsid sequences (region AD) were validated in six of the partner laboratories and provided correct genogroup typing information (GI or GII) when hybridized to the NoroChip v3.0. Results indicate that the current limiting factor for implementing the NoroChip v3.0 as a strain typing tool is the difficulty obtaining a long, specific amplicon from all circulating norovirus strains. Data obtained with the longer region AD amplicon provided the best discrimination between norovirus strains.

Original languageEnglish
Pages (from-to)233-250
Number of pages18
JournalJournal of Virological Methods
Volume173
Issue number2
DOIs
Publication statusPublished - 2011 May 1
Externally publishedYes

Keywords

  • Genotyping
  • Microarray
  • Norovirus

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Development and validation of a microarray for the confirmation and typing of norovirus RT-PCR products'. Together they form a unique fingerprint.

  • Cite this

    Mattison, K., Corneau, N., Berg, I., Bosch, A., Duizer, E., Gutiérrez-Aguirre, I., L'Homme, Y., Lucero, Y., Luo, Z., Martyres, A., Myrmel, M., O'Ryan, M., Pagotto, F., Sano, D., Svraka, S., Urzua, U., & Bidawid, S. (2011). Development and validation of a microarray for the confirmation and typing of norovirus RT-PCR products. Journal of Virological Methods, 173(2), 233-250. https://doi.org/10.1016/j.jviromet.2011.02.011