Development and evaluation of a new selective agar plate for penicillin-resistant pneumococci (PRSP)

Junichi Matsuda, Kazuyuki Sugawara, Ryoji Yoshida, Kazunori Tomono, Mitsuo Kaku

Research output: Contribution to journalArticlepeer-review

Abstract

Penicillin-resistant pneumococci (PRSP) have been increasing recently in our hospital. The percentages of penicillin-resistant strains in isolated pneumococci were 38.5% in 1992, 33% in 1993 and 34% in 1994. We developed a new selective agar plate for PRSP (PRSP-SM). Briefly, we added oxacillin (0.1 mg/1), aztreonam (10 mg/1) and gentamicin (5 mg/1) to Tripticase soy II agar supplemented with 5% sheep blood. In these agar plates, 100% (70/70) of PRP grew and 81% (91/112) of penicillin-sensitive pneumococci (PSSP) did not grow. However, some gram-negative rods (Pseudomonas aeruginosa, Pseudomonas cepacia, Serratia marcescens, Xanthomonas maltophilia) also grew. Antibiotics in the agar plates stored at 4°C were active enough for 42 days (oxacillin) or 90 days (aztreonam, gentamicin). In these agar plates, PRSP in the clinical samples grew by overnight incubation, however major contaminant organisms, P.aeruginosa, Haemophilus influenzae, Staphylococcus aureus, Branhamella catarrhalis and PSSP almost never grow.

Original languageEnglish
Pages (from-to)679-682
Number of pages4
JournalJapanese Journal of Chemotherapy
Volume43
Issue number7
DOIs
Publication statusPublished - 1995
Externally publishedYes

Keywords

  • Selective Medium
  • penicillin resistant S.pneumoniae

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

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