Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos

Brian S. Santos, Maria Rowena R. Romana-Eguia, Zubaida U. Basiao, Minoru Ikeda

Research output: Contribution to journalLetterpeer-review

5 Citations (Scopus)

Abstract

The milkfish, Chanos chanos, is an important aquaculture resource in Southeast Asia. Using NGS data, 72 microsatellite markers were developed. PCR product confirmation using agarose gel electrophoresis allowed the identification of 24 utilizable markers. Milkfish samples (n = 48) from a hatchery stock in Palawan, Philippines were analyzed for genetic variability at the aforementioned 24 loci. Consequently, nine of these microsatellite loci were noted to have high success rate in amplification, exhibited polymorphism with 19 maximum number of alleles and no null alleles. These are the first microsatellite markers to be developed and characterized for C. chanos that will efficiently enable genetic stock delineation and monitoring as well as marker-aided genetic improvement research.

Original languageEnglish
Pages (from-to)451-453
Number of pages3
JournalConservation Genetics Resources
Volume7
Issue number2
DOIs
Publication statusPublished - 2015 Jun 1

Keywords

  • Chanos chanos
  • Microsatellite markers
  • Milkfish
  • Population genetic structure

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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