Development and characterization of microsatellite markers for Veratrum Maackii (Melanthiaceae)

Yue Li, Satoshi Iijima, Masayuki Maki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Premise of the study: Microsatellite markers were developed in Veratrum maackii (Melanthiaceae) to assess the pattern of population genetic structures across the species' distribution. Methods and Results: Ten polymorphic loci were isolated from V. maackii using 454 shotgun pyrosequencing and the biotin capture method. The number of alleles per locus ranged from three to 19. The observed heterozygosity and expected heterozygosity ranged from 0.143 to 0.929 and 0.269 to 0.904, respectively. Conclusions: These newly developed microsatellite markers are useful for assessing the genetic diversity, population structure, and demographic history of V. maackii across its distribution range.

Original languageEnglish
Article number1500030
JournalApplications in Plant Sciences
Volume3
Issue number6
DOIs
Publication statusPublished - 2015 Jun

Keywords

  • 454 shotgun sequencing
  • Genetic structure
  • Melanthiaceae
  • Microsatellite
  • Multiplex PCR
  • Veratrum maackii.

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

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