TY - JOUR
T1 - Determination of urinary 8-epi-prostaglandin F(2α) using liquid chromatography-tandem mass spectrometry
T2 - Increased excretion in diabetics
AU - Murai, Yuriko
AU - Hishinuma, Takanori
AU - Suzuki, Naoto
AU - Satoh, Jo
AU - Toyota, Takayoshi
AU - Mizugaki, Michinao
PY - 2000/7/3
Y1 - 2000/7/3
N2 - Liquid chromatography-tandem mass spectrometry (LC/MS-MS) was applied to the quantitative analysis of urinary 8-epi-prostaglandin F(2α) (8-epi-PGF(2α)) level. 8-Epi-PGF(2α) and its internal standard, [2H4]-8-epi-PGF(2α), were extracted from urine by using a solid phase extraction cartridge and loaded to LC/MS-MS in selected reaction monitoring (SRM) mode. The standard curve showed good linearity in the range of 40 pg to 10 ng (r = 0.997). The accuracy of the added 8-epi-PGF(2α) ranged from 96.8 to 104.9% with a mean ± SD of 99.5 ± 2.5%. The average level ± SD of urinary 8-epi-PGF(2α) in 13 healthy volunteers (five women and eight men, 31 ± 7.4 years old) was 429.4 ± 149.6 pg/mg creatinine. The level of seven patients with noninsulin dependent diabetes mellitus (two women and five men, 40 ± 13.6 years old), 630.9 ± 275.6 pg/mg creatinine, was statistically higher than that of healthy volunteers (P < 0.05). This finding suggested that diabetics are in a highly oxidative condition. This simple and rapid LC/MS-MS method can be used to elucidate the pathophysiological feature of diabetes or for monitoring the curative effect. (C) 2000 Elsevier Science Inc.
AB - Liquid chromatography-tandem mass spectrometry (LC/MS-MS) was applied to the quantitative analysis of urinary 8-epi-prostaglandin F(2α) (8-epi-PGF(2α)) level. 8-Epi-PGF(2α) and its internal standard, [2H4]-8-epi-PGF(2α), were extracted from urine by using a solid phase extraction cartridge and loaded to LC/MS-MS in selected reaction monitoring (SRM) mode. The standard curve showed good linearity in the range of 40 pg to 10 ng (r = 0.997). The accuracy of the added 8-epi-PGF(2α) ranged from 96.8 to 104.9% with a mean ± SD of 99.5 ± 2.5%. The average level ± SD of urinary 8-epi-PGF(2α) in 13 healthy volunteers (five women and eight men, 31 ± 7.4 years old) was 429.4 ± 149.6 pg/mg creatinine. The level of seven patients with noninsulin dependent diabetes mellitus (two women and five men, 40 ± 13.6 years old), 630.9 ± 275.6 pg/mg creatinine, was statistically higher than that of healthy volunteers (P < 0.05). This finding suggested that diabetics are in a highly oxidative condition. This simple and rapid LC/MS-MS method can be used to elucidate the pathophysiological feature of diabetes or for monitoring the curative effect. (C) 2000 Elsevier Science Inc.
KW - 8-epi-PGF(2α)
KW - Determination
KW - Diabetes mellitus
KW - LC/MS-MS
KW - Prostaglandin
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U2 - 10.1016/S0090-6980(00)00061-7
DO - 10.1016/S0090-6980(00)00061-7
M3 - Article
C2 - 10938411
AN - SCOPUS:0034600757
VL - 62
SP - 173
EP - 181
JO - Prostaglandins and Other Lipid Mediators
JF - Prostaglandins and Other Lipid Mediators
SN - 0090-6980
IS - 2
ER -