Determination of urinary 12(S)-hydroxyeicosatetraenoic acid by liquid chromatography-tandem mass spectrometry with column-switching technique: Sex difference in healthy volunteers and patients with diabetes mellitus

Naoto Suzuki, Takanori Hishinuma, Toshihide Saga, Jo Sato, Takayoshi Toyota, Junichi Goto, Michinao Mizugaki

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We developed a determination method for human urinary 12-hydroxyeicosatetraenoic acid (12-HETE) using LC-MS-MS. This method, which includes simple extraction and detection in the SRM mode, allows precise and accurate determination of 12-HETE. There was a significant sex difference in urinary 12-HETE levels. Chiral analysis of 12-HETE using LC-MS-MS with column-switching technique revealed that the major enantiomer was 12(S)-HETE. Furthermore, the urinary level in patients with diabetes mellitus (DM) was analyzed. The present in vivo findings indicate that there could be difference in production of 12(S)-HETE between genders and 12(S)-HETE may play a role in the pathogenesis of DM.

Original languageEnglish
Pages (from-to)383-389
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume783
Issue number2
DOIs
Publication statusPublished - 2003 Jan 15

Keywords

  • 12(S)-Hydroxyeicosatetraenoic acid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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