Determination of the number of photoreactivating enzyme molecules per haploid saccharomyces cell

Akira Yasui, Wolfgang Laskowski

    Research output: Contribution to journalArticlepeer-review

    20 Citations (Scopus)

    Abstract

    Two haploid radiation-sensitive mutants of Saccharomyces were studied to investigate the formation of complex between photoreactivating-enzyme and substrate after ultra-violet irradiation. Using photo-flashes, the time necessary for maximum complex formation has been determined. Within 1 min, 70 per cent of the complexes have been formed. To determine the number of photoreactivating enzyme molecules per cell, the maximum dose decrement obtained after one photo-flash was determined and corrected for the effects of non-photoreactivable lesions. The corrected maximum dose decrement was found to be identical for both strains (8·5 erg mm-2). The number of photoreactivating-enzyme molecules involved in the photorepair of nuclear DNA damage was calculated as 272 ± 27.

    Original languageEnglish
    Pages (from-to)511-518
    Number of pages8
    JournalInternational Journal of Radiation Biology
    Volume28
    Issue number6
    DOIs
    Publication statusPublished - 1975

    ASJC Scopus subject areas

    • Radiological and Ultrasound Technology
    • Radiology Nuclear Medicine and imaging

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