Determination of the molecular nature and cellular localization of Thy-1 in human renal tissue

T. Miyata, K. Isobe, R. Dawson, M. A. Ritter, R. Inagi, O. Oda, R. Taguchi, H. Ikezawa, I. Inoue, H. Seo, M. Hasegawa, S. Kobayashi, K. Maeda, K. Yamada, I. Nakashima

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Abstract

The molecular nature and cellular localization of Thy-1 antigen in human renal tissue were studied. Strong immunohistochemical staining was observed in frozen sections of human kidney using monoclonal anti-human Thy-1 antibody; this reaction was almost completely abolished by pretreating the kidney section with phosphatidyl inositol (PI)-specific phospholipase C (PI-PLC). Immunohistochemical analysis revealed that the Thy-1 antigen is localized on the proximal tubular epithelial cells and the Bowman's capsule of the glomerulus. Northern blot analysis of renal mRNA using a cloned human Thy-1 gene revealed the presence of human Thy-1 mRNA of a similar size to the one in human brain. When a human kidney cDNA library was screened with the same probe, a cDNA of human Thy-1 was isolated. Moreover, human Thy-1 protein with a molecular weight (MW) of 21,000 was detected in renal tissue by gel electrophoresis and Western blot analysis using monoclonal anti-human Thy-1 antibody. These data demonstrate for the first time the production of human Thy-1 as a PI-anchored protein with a unique cellular location in human renal tissue.

Original languageEnglish
Pages (from-to)391-395
Number of pages5
JournalImmunology
Volume69
Issue number3
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Miyata, T., Isobe, K., Dawson, R., Ritter, M. A., Inagi, R., Oda, O., Taguchi, R., Ikezawa, H., Inoue, I., Seo, H., Hasegawa, M., Kobayashi, S., Maeda, K., Yamada, K., & Nakashima, I. (1990). Determination of the molecular nature and cellular localization of Thy-1 in human renal tissue. Immunology, 69(3), 391-395.