Determination of the Degree of Acetylation of Chitin/Chitosan by Pyrolysis-Gas Chromatography in the Presence of Oxalic Acid

Hiroaki Sato, Shin Ichi Mizutani, Shin Tsuge, Hajime Ohtani, Keigo Aoi, Akinori Takasu, Masahiko Okada, Shiro Kobayashi, Toshitsugu Kiyosada, Shin Ichiro Shoda

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Abstract

A new method to determine directly and rapidly the degree of acetylation of chitin/chitosan was developed based on reactive pyrolysis-gas chromatography in the presence of an oxalic acid aqueous solution. The degree of acetylation was precisely evaluated on the basis of peak intensities of the characteristic products such as acetonitrile, acetic acid, and acetamide originating from the N-acetyl group of N-acetyl-D-glucosamine units of chitin/ chitosan. The observed values were in good agreement with those obtained by 1H NMR and the other methods. Moreover, the proposed technique was applicable to any kinds of chitin/chitosan samples over the whole range of acetylation including insoluble chitin/chitosan and perfectly acetylated artificial chitin having higher crystallinity to which 1H NMR had been inapplicable.

Original languageEnglish
Pages (from-to)7-12
Number of pages6
JournalAnalytical Chemistry
Volume70
Issue number1
DOIs
Publication statusPublished - 1998 Jan 1

ASJC Scopus subject areas

  • Analytical Chemistry

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    Sato, H., Mizutani, S. I., Tsuge, S., Ohtani, H., Aoi, K., Takasu, A., Okada, M., Kobayashi, S., Kiyosada, T., & Shoda, S. I. (1998). Determination of the Degree of Acetylation of Chitin/Chitosan by Pyrolysis-Gas Chromatography in the Presence of Oxalic Acid. Analytical Chemistry, 70(1), 7-12. https://doi.org/10.1021/ac9706685