Determination of phosphatidylcholine hydroperoxide in human plasma by chemiluminescence - high performance liquid chromatography

Teruo Miyazawa, Keiichi Yasuda, Kenshiro Fujimoto, Takashi Kaneda

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

To determine phosphatidylcholine hydroperoxide (PCOOH) in human plasma lipids, a chemiluminescence - high performance liquid chromatography (CL-HPLC)system was newly developed and applied. This system consisted of separation of phosphatidylcholine derivatives with normal phase HPLC and detection of hydroperoxide-specific chemiluminescence of PCOOH. By use of this system, the PCOOH in normal human plasma was quantitatively assayed to be 28–431 pmol hydroperoxide-02 per ml of plasma. The high sensitivities, reproducibilities and specificities of the CL-HPLC assay were shown for determining plasma PCOOH.

Original languageEnglish
Pages (from-to)1033-1044
Number of pages12
JournalAnalytical Letters
Volume21
Issue number6
DOIs
Publication statusPublished - 1988 Jun 1

Keywords

  • Chemiluminescence
  • HPLC
  • human plasma
  • phosphatidylcholine hydroperoxide

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

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