Determination of organophosphorus pesticides in biological samples of acute poisoning by HPLC with diode-array detector

Yuko Cho, Nancy Matsuoka, Akira Kamiya

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We have developed a simple and rapid method for measuring 11 organophosphorus pesticides (dichlorvos, methidathion, salithion, malathion, fenitrothion, fenthion, parathion, diazinon, ethylthiometon, O-ethyl O-(4- nitrophenyl)phenylphosphonothioate (EPN) and chlorpyrifos) and one metabolite (3-methyl-4-nitrophenol) of fenitrothion in serum and urine of acute poisoning patients by HPLC with a diode-array detector. An aliquot of the biological sample after deproteinization by acetonitrile was injected into a C18 column using acetonitrile-water as a mobile phase. The detection limits in serum and urine ranged from 0.05 to 6.8 μg/ml at a wavelength of 230 nm. This method was successfully applied to two actual cases of acute poisoning.

Original languageEnglish
Pages (from-to)737-740
Number of pages4
JournalChemical and Pharmaceutical Bulletin
Volume45
Issue number4
DOIs
Publication statusPublished - 1997 Apr
Externally publishedYes

Keywords

  • HPLC diode-array
  • acute poisoning
  • organophosphorus pesticide
  • serum
  • urine

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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