Abstract
A sensitive fluorimetric method for the determination of octopine, a member of opine family, is presented. The method is based on the formation of a fluorescent derivative of octopine with benzoin and the separation by high performance liquid chromatography using a reversed-phase column (Kaseisorb LC ODS-300) within 20 min. The octopine derivative is completely separated from other guanidino compounds including arginine which is generally very high in marine invertebrates. This method gives higher sensitivity, 5 pmol minimum detection, and better reproducibility than the electrophoresis method and colorimetric method.
| Original language | English |
|---|---|
| Pages (from-to) | 1035-1039 |
| Number of pages | 5 |
| Journal | Biochemistry international |
| Volume | 23 |
| Issue number | 6 |
| Publication status | Published - 1991 Jul 26 |
ASJC Scopus subject areas
- Biochemistry