Determination of octopine by pre-column fluorescence derivatization using benzoin

Minoru Sato, M. Takeuchi, N. Kanno, E. Nagahisa, Y. Sato

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A sensitive fluorimetric method for the determination of octopine, a member of opine family, is presented. The method is based on the formation of a fluorescent derivative of octopine with benzoin and the separation by high performance liquid chromatography using a reversed-phase column (Kaseisorb LC ODS-300) within 20 min. The octopine derivative is completely separated from other guanidino compounds including arginine which is generally very high in marine invertebrates. This method gives higher sensitivity, 5 pmol minimum detection, and better reproducibility than the electrophoresis method and colorimetric method.

Original languageEnglish
Pages (from-to)1035-1039
Number of pages5
JournalBiochemistry international
Volume23
Issue number6
Publication statusPublished - 1991 Jul 26

ASJC Scopus subject areas

  • Biochemistry

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