Determination of leukotriene E4 in human urine using liquid chromatography-tandem mass spectrometry

Michinao Mizugaki; Takanori Hishinuma; Naoto Suzuki

doi:10.1016/S0378-4347(99)00174-7

Determination of leukotriene E₄ in human urine using liquid chromatography-tandem mass spectrometry

Michinao Mizugaki, Takanori Hishinuma, Naoto Suzuki

PHA - Pharmacy

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

A liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed for the quantitation of urinary leukotriene E₄ (LTE₄). LTE₄ and its internal standard were extracted by solid-phase extraction and analysed using LC-MS-MS in the selected reaction monitoring (SRM) mode. A good linear response over the range of 10 pg to 10 ng was demonstrated. The accuracy of added LTE₄ ranged from 97.0% to 108.0% with a mean and SD of 100.6±2.4%. We detected LTE₄ (63.1±18.7 pg/mg creatinine, n=10) in healthy human urine. This method can be used to determine LTE₄ in biological samples. Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	279-285
Number of pages	7
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	729
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-4347(99)00174-7
Publication status	Published - 1999 Jun 11

Keywords

Leukotrienes

ASJC Scopus subject areas

Chemistry(all)

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title = "Determination of leukotriene E4 in human urine using liquid chromatography-tandem mass spectrometry",

abstract = "A liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed for the quantitation of urinary leukotriene E4 (LTE4). LTE4 and its internal standard were extracted by solid-phase extraction and analysed using LC-MS-MS in the selected reaction monitoring (SRM) mode. A good linear response over the range of 10 pg to 10 ng was demonstrated. The accuracy of added LTE4 ranged from 97.0% to 108.0% with a mean and SD of 100.6±2.4%. We detected LTE4 (63.1±18.7 pg/mg creatinine, n=10) in healthy human urine. This method can be used to determine LTE4 in biological samples. Copyright (C) 1999 Elsevier Science B.V.",

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author = "Michinao Mizugaki and Takanori Hishinuma and Naoto Suzuki",

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T1 - Determination of leukotriene E4 in human urine using liquid chromatography-tandem mass spectrometry

AU - Mizugaki, Michinao

AU - Hishinuma, Takanori

AU - Suzuki, Naoto

PY - 1999/6/11

Y1 - 1999/6/11

N2 - A liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed for the quantitation of urinary leukotriene E4 (LTE4). LTE4 and its internal standard were extracted by solid-phase extraction and analysed using LC-MS-MS in the selected reaction monitoring (SRM) mode. A good linear response over the range of 10 pg to 10 ng was demonstrated. The accuracy of added LTE4 ranged from 97.0% to 108.0% with a mean and SD of 100.6±2.4%. We detected LTE4 (63.1±18.7 pg/mg creatinine, n=10) in healthy human urine. This method can be used to determine LTE4 in biological samples. Copyright (C) 1999 Elsevier Science B.V.

AB - A liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed for the quantitation of urinary leukotriene E4 (LTE4). LTE4 and its internal standard were extracted by solid-phase extraction and analysed using LC-MS-MS in the selected reaction monitoring (SRM) mode. A good linear response over the range of 10 pg to 10 ng was demonstrated. The accuracy of added LTE4 ranged from 97.0% to 108.0% with a mean and SD of 100.6±2.4%. We detected LTE4 (63.1±18.7 pg/mg creatinine, n=10) in healthy human urine. This method can be used to determine LTE4 in biological samples. Copyright (C) 1999 Elsevier Science B.V.

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