Determination of leukotriene E4 in human urine using liquid chromatography-tandem mass spectrometry

Michinao Mizugaki, Takanori Hishinuma, Naoto Suzuki

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)


A liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed for the quantitation of urinary leukotriene E4 (LTE4). LTE4 and its internal standard were extracted by solid-phase extraction and analysed using LC-MS-MS in the selected reaction monitoring (SRM) mode. A good linear response over the range of 10 pg to 10 ng was demonstrated. The accuracy of added LTE4 ranged from 97.0% to 108.0% with a mean and SD of 100.6±2.4%. We detected LTE4 (63.1±18.7 pg/mg creatinine, n=10) in healthy human urine. This method can be used to determine LTE4 in biological samples. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)279-285
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Issue number1-2
Publication statusPublished - 1999 Jun 11


  • Leukotrienes

ASJC Scopus subject areas

  • Chemistry(all)

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