TY - JOUR
T1 - Determination of intestinal disappearance of lysine and methionine derived from ruminally protected lysine and methionine in holstein heifers
AU - Watanabe, K.
AU - Sato, Hiroyuki
AU - Kobayashi, T.
AU - Katoh, K.
AU - Obara, Y.
PY - 2003/4/1
Y1 - 2003/4/1
N2 - In order to estimate the availability of ruminally-protected Lys (RPLys) anti Met (RPMet), quantitative changes of free Lys and Met in the total abomasal outflow and feces were determined in three Holstein heifers with a T-shaped duodenal cannula. RPLys was prepared by coating Lys with fat and RPMet with a pH-sensitive polymer. RPLys and RPMet containing 30 g of each amino acid were supplied together to heifers, and total abomasal outflow was collected hourly from the T-shaped duodenal cannula for a 48 h period. Collected abomasal outflows were fractionated into liquid and solid phases to measure free Lys and Met concentration. At 2 wk after total abomasal outflow sampling, heifers were supplied RPLys and RPMet together again, and total feces were collected every 12 h for a 3 d period to estimate excreted RPLys and RPMet in feces. The amounts of Lys and Met recovered from the liquid fractions of abomasal outflow were estimated to be 23.9±8.3% and 68.6±3.6% (p=0.008) of RPLys and RPMet ingested, respectively. Final intestinal disappearances of Lys and Met were estimated to be 49.5±2.6% and 78.2%±6.5% (p=0.015) of ingested RPLys and RPMet, respectively.
AB - In order to estimate the availability of ruminally-protected Lys (RPLys) anti Met (RPMet), quantitative changes of free Lys and Met in the total abomasal outflow and feces were determined in three Holstein heifers with a T-shaped duodenal cannula. RPLys was prepared by coating Lys with fat and RPMet with a pH-sensitive polymer. RPLys and RPMet containing 30 g of each amino acid were supplied together to heifers, and total abomasal outflow was collected hourly from the T-shaped duodenal cannula for a 48 h period. Collected abomasal outflows were fractionated into liquid and solid phases to measure free Lys and Met concentration. At 2 wk after total abomasal outflow sampling, heifers were supplied RPLys and RPMet together again, and total feces were collected every 12 h for a 3 d period to estimate excreted RPLys and RPMet in feces. The amounts of Lys and Met recovered from the liquid fractions of abomasal outflow were estimated to be 23.9±8.3% and 68.6±3.6% (p=0.008) of RPLys and RPMet ingested, respectively. Final intestinal disappearances of Lys and Met were estimated to be 49.5±2.6% and 78.2%±6.5% (p=0.015) of ingested RPLys and RPMet, respectively.
KW - Heifer
KW - Intestinal Disappearance
KW - Ruminally Protected Lysine
KW - Ruminally Protected Met
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U2 - 10.5713/ajas.2003.549
DO - 10.5713/ajas.2003.549
M3 - Article
AN - SCOPUS:0037384214
VL - 16
SP - 549
EP - 554
JO - Asian-Australasian Journal of Animal Sciences
JF - Asian-Australasian Journal of Animal Sciences
SN - 1011-2367
IS - 4
ER -