Determination of iminosugars in mulberry leaves and silkworms using hydrophilic interaction chromatography-tandem mass spectrometry

Kiyotaka Nakagawa, Kenta Ogawa, Ohki Higuchi, Toshiyuki Kimura, Teruo Miyazawa, Masatoshi Hori

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

Mulberry 1-deoxynojirimycin (DNJ, a potent α-glycosidase inhibitor) has been investigated thoroughly for its analytical methods and therapeutic potential against diabetes, whereas little attention has been given to other iminosugars such as 2-O-α-d-galactopyranosyl-DNJ (GAL-DNJ) and fagomine. For instance, concentration and composition of these iminosugars in mulberry leaves as well as sericulture products have not been fully characterized due to lack of suitable analytical methods. Here we developed a simultaneous determination method for DNJ, GAL-DNJ, and fagomine using hydrophilic interaction chromatography (HILIC) with tandem mass spectrometry (MS/MS). When mulberry leaf extracts were subjected to HILIC-MS/MS with multiple reaction monitoring (MRM), individual iminosugars could be separated and detected. The developed method is sufficiently sensitive for determining iminosugars in mulberry leaves as well as silkworms, providing new information (e.g., different amounts of iminosugars in mulberry leaf varieties; high DNJ and low GAL-DNJ in the silkworm body, especially in the blood) that is useful for producing iminosugar-rich products for nutraceutical purposes.

Original languageEnglish
Pages (from-to)217-222
Number of pages6
JournalAnalytical Biochemistry
Volume404
Issue number2
DOIs
Publication statusPublished - 2010 Sep

Keywords

  • 1-Deoxynojirimycin
  • Diabetes
  • HILIC-MS/MS
  • Iminosugars
  • Mulberry leaves
  • Silkworms

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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