Determination of critical epitope of PcMab-47 against human podocalyxin

Shunsuke Itai, Shinji Yamada, Mika K. Kaneko, Yukinari Kato

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Podocalyxin (PODXL) is a type I transmembrane protein, which is highly glycosylated. PODXL is expressed in some types of human cancer tissues including oral, breast, and lung cancer tissues and may promote tumor growth, invasion, and metastasis. We previously produced PcMab-47, a novel anti-PODXL monoclonal antibody (mAb) which reacts with endogenous PODXL-expressing cancer cell lines and normal cells independently of glycosylation in Western blot, flow cytometry, and immunohistochemical analysis. In this study, we used enzyme-linked immunosorbent assay (ELISA), flow cytometry, and immunohistochemical analysis to determine the epitope of PcMab-47. The minimum epitope of PcMab-47 was found to be Asp207, His208, Leu209, and Met210. A blocking peptide containing this minimum epitope completely neutralized PcMab-47 reaction against oral cancer cells by flow cytometry and immunohistochemical analysis. These findings could lead to the production of more functional anti-PODXL mAbs, which are advantageous for antitumor activities.

Original languageEnglish
Pages (from-to)78-82
Number of pages5
JournalBiochemistry and Biophysics Reports
Volume14
DOIs
Publication statusPublished - 2018 Jul

Keywords

  • Epitope mapping
  • Monoclonal antibody
  • Oral cancer
  • PODXL
  • Podocalyxin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Fingerprint Dive into the research topics of 'Determination of critical epitope of PcMab-47 against human podocalyxin'. Together they form a unique fingerprint.

Cite this