Determination of chlorhexidine (CHD) and nonylphenolethoxylates (NPEOn) using LC-ESI-MS method and application to hemolyzed blood

Kiyotaka Usui, Takanori Hishinuma, Hiroaki Yamaguchi, Naoki Tachiiri, Junichi Goto

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Rapid and reliable methods for identification of chlorhexidine (CHD) and nonylphenolethoxylates (NPEOn) in antiseptic and hemolyzed blood using electrospray ionization mass spectrometry (ESI-MS) were developed. Fragmental analysis provides accurate evidence for the presence of CHD in the samples. For the determination of CHD in hemolyzed blood, the method was also developed using LC-ESI-MS. Linearity of calibration curve was obtained over the concentration range of 0.1-11 μg/mL with residuals from -4.3 to 6.7%. We applied the methods to the case of suicidal injection of antiseptic and successfully detected CHD and NPEOn from hemolyzed blood. The CHD concentration was 352 μg/mL.

Original languageEnglish
Pages (from-to)105-109
Number of pages5
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume831
Issue number1-2
DOIs
Publication statusPublished - 2006 Feb 2

Keywords

  • Antiseptic
  • Blood
  • Chlorhexidine
  • Disinfectant
  • Forensic medicine
  • Forensic science
  • Human
  • LC-ESI
  • LC-ESI-MS
  • Nonylphenolethoxylates
  • Suicide
  • Toxicology

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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