Determination of captopril in human blood by high-performance liquid chromatography with electrochemical detection

Kazutake Shimada, Makoto Tanaka, Toshio Nambara, Yutaka Imai, Keishi Abe, Kaoru Yoshinaga

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)

Abstract

A new method for quantitation of captopril in human blood is described. Captopril was derivatized with N-(4-dimethylaminophenyl)maleimide into the electrochemically active adduct. The derivative was separated and determined by high-performance liquid chromatography with an electrochemical detector on a reversed-phase column. The proposed method was satisfactory for determination of captopril in whole blood with respect to accuracy and precision. The detection limit of captopril thereby obtained was 10 ng/ml. The blood levels of captopril in patients orally given an officinal dose were measured by the present method.

Original languageEnglish
Pages (from-to)445-451
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume227
Issue number2
DOIs
Publication statusPublished - 1982 Feb 12

ASJC Scopus subject areas

  • Chemistry(all)

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