Determination of captopril in human blood by high-performance liquid chromatography with electrochemical detection

Kazutake Shimada; Makoto Tanaka; Toshio Nambara; Yutaka Imai; Keishi Abe; Kaoru Yoshinaga

doi:10.1016/S0378-4347(00)80397-7

Determination of captopril in human blood by high-performance liquid chromatography with electrochemical detection

Kazutake Shimada, Makoto Tanaka, Toshio Nambara, Yutaka Imai, Keishi Abe, Kaoru Yoshinaga

Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

60 Citations (Scopus)

Abstract

A new method for quantitation of captopril in human blood is described. Captopril was derivatized with N-(4-dimethylaminophenyl)maleimide into the electrochemically active adduct. The derivative was separated and determined by high-performance liquid chromatography with an electrochemical detector on a reversed-phase column. The proposed method was satisfactory for determination of captopril in whole blood with respect to accuracy and precision. The detection limit of captopril thereby obtained was 10 ng/ml. The blood levels of captopril in patients orally given an officinal dose were measured by the present method.

Original language	English
Pages (from-to)	445-451
Number of pages	7
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	227
Issue number	2
DOIs	https://doi.org/10.1016/S0378-4347(00)80397-7
Publication status	Published - 1982 Feb 12

ASJC Scopus subject areas

Chemistry(all)

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Determination of captopril in human blood by high-performance liquid chromatography with electrochemical detection. / Shimada, Kazutake; Tanaka, Makoto; Nambara, Toshio; Imai, Yutaka; Abe, Keishi; Yoshinaga, Kaoru.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 227, No. 2, 12.02.1982, p. 445-451.

Research output: Contribution to journal › Article › peer-review

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abstract = "A new method for quantitation of captopril in human blood is described. Captopril was derivatized with N-(4-dimethylaminophenyl)maleimide into the electrochemically active adduct. The derivative was separated and determined by high-performance liquid chromatography with an electrochemical detector on a reversed-phase column. The proposed method was satisfactory for determination of captopril in whole blood with respect to accuracy and precision. The detection limit of captopril thereby obtained was 10 ng/ml. The blood levels of captopril in patients orally given an officinal dose were measured by the present method.",

author = "Kazutake Shimada and Makoto Tanaka and Toshio Nambara and Yutaka Imai and Keishi Abe and Kaoru Yoshinaga",

note = "Funding Information: Insurance Hospital, for his support throughout this work. This work was supported in part by a grant from the iMinistry of Education, Science and Culture, Japan.",

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AU - Abe, Keishi

AU - Yoshinaga, Kaoru

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AB - A new method for quantitation of captopril in human blood is described. Captopril was derivatized with N-(4-dimethylaminophenyl)maleimide into the electrochemically active adduct. The derivative was separated and determined by high-performance liquid chromatography with an electrochemical detector on a reversed-phase column. The proposed method was satisfactory for determination of captopril in whole blood with respect to accuracy and precision. The detection limit of captopril thereby obtained was 10 ng/ml. The blood levels of captopril in patients orally given an officinal dose were measured by the present method.

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