Determination of Amino Acids By High-Performance Liquid Chromatography with Electrochemical Detection Using Ferrocene Derivatization Reagents

Kazutake Shimada; Yukinori Kawai; Tomoyuki Oe

doi:10.1080/01483918908051740

Determination of Amino Acids By High-Performance Liquid Chromatography with Electrochemical Detection Using Ferrocene Derivatization Reagents

Kazutake Shimada, Yukinori Kawai, Tomoyuki Oe

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Two new reagents possessing ferrocene as an electrophore and isothiocyanate reactive toward the amino group were prepared and evaluated for pre-column derivatization of amino acids in highperformance liquid chromatography with electrochemical detection. The utilities of these reagents were investigated employing glycine as a model compound. Ferrocenylisothiocyanate was more favorable with respect to reactivity and electrochemical properties. The newly developed method was applied to the determination of 4-aminobutyric acid in biological specimens.

Original language	English
Pages (from-to)	359-371
Number of pages	13
Journal	Journal of Liquid Chromatography
Volume	12
Issue number	3
DOIs	https://doi.org/10.1080/01483918908051740
Publication status	Published - 1989 Mar 1
Externally published	Yes

ASJC Scopus subject areas

Molecular Medicine

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title = "Determination of Amino Acids By High-Performance Liquid Chromatography with Electrochemical Detection Using Ferrocene Derivatization Reagents",

abstract = "Two new reagents possessing ferrocene as an electrophore and isothiocyanate reactive toward the amino group were prepared and evaluated for pre-column derivatization of amino acids in highperformance liquid chromatography with electrochemical detection. The utilities of these reagents were investigated employing glycine as a model compound. Ferrocenylisothiocyanate was more favorable with respect to reactivity and electrochemical properties. The newly developed method was applied to the determination of 4-aminobutyric acid in biological specimens.",

author = "Kazutake Shimada and Yukinori Kawai and Tomoyuki Oe",

note = "Funding Information: ACKNOWLEDGEMENTS The authors are indebted to the staff of the central analytical laboratory of this Faculty for spectral measurements. Thanks are also due to Dr. H. Iwase (Ajinomoto Co.) for supplying amino acids. This work was supported, in part, by a grant from Ministry of Education, Science and Culture of Japan.",

year = "1989",

month = mar,

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doi = "10.1080/01483918908051740",

language = "English",

volume = "12",

pages = "359--371",

journal = "Journal of Liquid Chromatography and Related Technologies",

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T1 - Determination of Amino Acids By High-Performance Liquid Chromatography with Electrochemical Detection Using Ferrocene Derivatization Reagents

AU - Shimada, Kazutake

AU - Kawai, Yukinori

AU - Oe, Tomoyuki

N1 - Funding Information: ACKNOWLEDGEMENTS The authors are indebted to the staff of the central analytical laboratory of this Faculty for spectral measurements. Thanks are also due to Dr. H. Iwase (Ajinomoto Co.) for supplying amino acids. This work was supported, in part, by a grant from Ministry of Education, Science and Culture of Japan.

PY - 1989/3/1

Y1 - 1989/3/1

N2 - Two new reagents possessing ferrocene as an electrophore and isothiocyanate reactive toward the amino group were prepared and evaluated for pre-column derivatization of amino acids in highperformance liquid chromatography with electrochemical detection. The utilities of these reagents were investigated employing glycine as a model compound. Ferrocenylisothiocyanate was more favorable with respect to reactivity and electrochemical properties. The newly developed method was applied to the determination of 4-aminobutyric acid in biological specimens.

AB - Two new reagents possessing ferrocene as an electrophore and isothiocyanate reactive toward the amino group were prepared and evaluated for pre-column derivatization of amino acids in highperformance liquid chromatography with electrochemical detection. The utilities of these reagents were investigated employing glycine as a model compound. Ferrocenylisothiocyanate was more favorable with respect to reactivity and electrochemical properties. The newly developed method was applied to the determination of 4-aminobutyric acid in biological specimens.

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Determination of Amino Acids By High-Performance Liquid Chromatography with Electrochemical Detection Using Ferrocene Derivatization Reagents

Abstract

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