Determination of Amino Acids By High-Performance Liquid Chromatography with Electrochemical Detection Using Ferrocene Derivatization Reagents

Kazutake Shimada; Yukinori Kawai; Tomoyuki Oe

doi:10.1080/01483918908051740

Determination of Amino Acids By High-Performance Liquid Chromatography with Electrochemical Detection Using Ferrocene Derivatization Reagents

Kazutake Shimada, Yukinori Kawai, Tomoyuki Oe

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Two new reagents possessing ferrocene as an electrophore and isothiocyanate reactive toward the amino group were prepared and evaluated for pre-column derivatization of amino acids in highperformance liquid chromatography with electrochemical detection. The utilities of these reagents were investigated employing glycine as a model compound. Ferrocenylisothiocyanate was more favorable with respect to reactivity and electrochemical properties. The newly developed method was applied to the determination of 4-aminobutyric acid in biological specimens.

Original language	English
Pages (from-to)	359-371
Number of pages	13
Journal	Journal of Liquid Chromatography
Volume	12
Issue number	3
DOIs	https://doi.org/10.1080/01483918908051740
Publication status	Published - 1989 Mar 1
Externally published	Yes

ASJC Scopus subject areas

Molecular Medicine

Access to Document

10.1080/01483918908051740

Fingerprint Dive into the research topics of 'Determination of Amino Acids By High-Performance Liquid Chromatography with Electrochemical Detection Using Ferrocene Derivatization Reagents'. Together they form a unique fingerprint.

Cite this

@article{61168718b2fd4d0590e3a519fbdd977f,

title = "Determination of Amino Acids By High-Performance Liquid Chromatography with Electrochemical Detection Using Ferrocene Derivatization Reagents",

abstract = "Two new reagents possessing ferrocene as an electrophore and isothiocyanate reactive toward the amino group were prepared and evaluated for pre-column derivatization of amino acids in highperformance liquid chromatography with electrochemical detection. The utilities of these reagents were investigated employing glycine as a model compound. Ferrocenylisothiocyanate was more favorable with respect to reactivity and electrochemical properties. The newly developed method was applied to the determination of 4-aminobutyric acid in biological specimens.",

author = "Kazutake Shimada and Yukinori Kawai and Tomoyuki Oe",

year = "1989",

month = mar,

day = "1",

doi = "10.1080/01483918908051740",

language = "English",

volume = "12",

pages = "359--371",

journal = "Journal of Liquid Chromatography and Related Technologies",

issn = "1082-6076",

publisher = "Taylor and Francis Ltd.",

number = "3",

}

TY - JOUR

T1 - Determination of Amino Acids By High-Performance Liquid Chromatography with Electrochemical Detection Using Ferrocene Derivatization Reagents

AU - Shimada, Kazutake

AU - Kawai, Yukinori

AU - Oe, Tomoyuki

PY - 1989/3/1

Y1 - 1989/3/1

N2 - Two new reagents possessing ferrocene as an electrophore and isothiocyanate reactive toward the amino group were prepared and evaluated for pre-column derivatization of amino acids in highperformance liquid chromatography with electrochemical detection. The utilities of these reagents were investigated employing glycine as a model compound. Ferrocenylisothiocyanate was more favorable with respect to reactivity and electrochemical properties. The newly developed method was applied to the determination of 4-aminobutyric acid in biological specimens.

AB - Two new reagents possessing ferrocene as an electrophore and isothiocyanate reactive toward the amino group were prepared and evaluated for pre-column derivatization of amino acids in highperformance liquid chromatography with electrochemical detection. The utilities of these reagents were investigated employing glycine as a model compound. Ferrocenylisothiocyanate was more favorable with respect to reactivity and electrochemical properties. The newly developed method was applied to the determination of 4-aminobutyric acid in biological specimens.

UR - http://www.scopus.com/inward/record.url?scp=0024582905&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024582905&partnerID=8YFLogxK

U2 - 10.1080/01483918908051740

DO - 10.1080/01483918908051740

M3 - Article

AN - SCOPUS:0024582905

VL - 12

SP - 359

EP - 371

JO - Journal of Liquid Chromatography and Related Technologies

JF - Journal of Liquid Chromatography and Related Technologies

SN - 1082-6076

IS - 3

ER -