Determination of a new antihypertensive agent (2R,4R)‐2‐(2‐Hydroxyphenyl)‐3‐(3‐mercaptopropionyl)‐4‐thiazolidinecarboxylic Acid in Blood by High‐Performance Liquid Chromatography with Electrochemical Detection

Kazutake Shimada, Makoto Tanaka, Toshio Nambara, Yutaka Imai

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A sensitive method has been developed for the determination of a new antihypertensive agent, (2R,4R)‐2‐(2‐hydroxyphenyl)‐3‐(3‐mercaptopropionyl)‐4‐thiazolidinecarboxylic acid (I), in human blood. Because the drug is unstable in biological fluids, it was immediately derivatized by treating the freshly drawn blood specimens with N‐(4‐anilinophenyl)maleimide. The adduct was separated and determined by high‐performance liquid chromatography with electrochemical detection on a reverse‐phase column. The assay method was satisfactory with respect to the sensitivity and precision, providing a quantitation limit of 2 ng/ml and coefficient of variation of 3%. Preliminary pharmacokinetic data were obtained by orally administering I to two patients with essential hypertension.

Original languageEnglish
Pages (from-to)119-121
Number of pages3
JournalJournal of Pharmaceutical Sciences
Volume73
Issue number1
DOIs
Publication statusPublished - 1984 Jan

Keywords

  • (2R,4R)‐2‐(2‐Hydroxyphenyl)‐3‐(3‐mercaptopropionyl)‐4‐thiazolidinecarboxylic acid—determination in human blood, high‐performance liquid chromatography with electrochemical detection
  • Antihypertensive agents—(2R,4R)‐2‐(2‐hydroxyphenyl)‐3‐(3‐mercaptopropionyl)‐4‐thiazolidinecarboxylic acid, determination in human blood, high‐performance liquid chromatography with electrochemical detection
  • High‐performance liquid chromatography—determination of (2R,4R)‐2‐(2‐hydroxyphenyl)‐3‐(3‐mercaptopropionyl)‐4‐thiazolidinecarboxylic acid in human blood, electrochemical detection

ASJC Scopus subject areas

  • Pharmaceutical Science

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