Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection

Toshiyuki Kimura; Kiyotaka Nakagawa; Yuko Saito; Kenji Yamagishi; Masahiro Suzuki; Kohji Yamaki; Hiroshi Shinmoto; Teruo Miyazawa

doi:10.1021/jf0306901

Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection

Toshiyuki Kimura, Kiyotaka Nakagawa, Yuko Saito, Kenji Yamagishi, Masahiro Suzuki, Kohji Yamaki, Hiroshi Shinmoto, Teruo Miyazawa

Research output: Contribution to journal › Article › peer-review

76 Citations (Scopus)

Abstract

A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.

Original language	English
Pages (from-to)	1415-1418
Number of pages	4
Journal	Journal of Agricultural and Food Chemistry
Volume	52
Issue number	6
DOIs	https://doi.org/10.1021/jf0306901
Publication status	Published - 2004 Mar 24

Keywords

1-Deoxynojirimycin
Evaporative light scattering detector
Morus alba
Mulberry leaves

ASJC Scopus subject areas

Chemistry(all)
Agricultural and Biological Sciences(all)

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10.1021/jf0306901

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Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection. / Kimura, Toshiyuki; Nakagawa, Kiyotaka; Saito, Yuko; Yamagishi, Kenji; Suzuki, Masahiro; Yamaki, Kohji; Shinmoto, Hiroshi; Miyazawa, Teruo.

In: Journal of Agricultural and Food Chemistry, Vol. 52, No. 6, 24.03.2004, p. 1415-1418.

Research output: Contribution to journal › Article › peer-review

Kimura, Toshiyuki ; Nakagawa, Kiyotaka ; Saito, Yuko ; Yamagishi, Kenji ; Suzuki, Masahiro ; Yamaki, Kohji ; Shinmoto, Hiroshi ; Miyazawa, Teruo. / Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection. In: Journal of Agricultural and Food Chemistry. 2004 ; Vol. 52, No. 6. pp. 1415-1418.

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abstract = "A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.",

keywords = "1-Deoxynojirimycin, Evaporative light scattering detector, Morus alba, Mulberry leaves",

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AU - Saito, Yuko

AU - Yamagishi, Kenji

AU - Suzuki, Masahiro

AU - Yamaki, Kohji

AU - Shinmoto, Hiroshi

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