Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection

Toshiyuki Kimura, Kiyotaka Nakagawa, Yuko Saito, Kenji Yamagishi, Masahiro Suzuki, Kohji Yamaki, Hiroshi Shinmoto, Teruo Miyazawa

Research output: Contribution to journalArticlepeer-review

76 Citations (Scopus)

Abstract

A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.

Original languageEnglish
Pages (from-to)1415-1418
Number of pages4
JournalJournal of Agricultural and Food Chemistry
Volume52
Issue number6
DOIs
Publication statusPublished - 2004 Mar 24

Keywords

  • 1-Deoxynojirimycin
  • Evaporative light scattering detector
  • Morus alba
  • Mulberry leaves

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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