Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection

Toshiyuki Kimura; Kiyotaka Nakagawa; Yuko Saito; Kenji Yamagishi; Masahiro Suzuki; Kohji Yamaki; Hiroshi Shinmoto; Teruo Miyazawa

doi:10.1021/jf0306901

Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection

Toshiyuki Kimura, Kiyotaka Nakagawa, Yuko Saito, Kenji Yamagishi, Masahiro Suzuki, Kohji Yamaki, Hiroshi Shinmoto, Teruo Miyazawa

New Industry Creation Hatchery Center (NICHe)

Research output: Contribution to journal › Article › peer-review

83 Citations (Scopus)

Abstract

A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.

Original language	English
Pages (from-to)	1415-1418
Number of pages	4
Journal	Journal of Agricultural and Food Chemistry
Volume	52
Issue number	6
DOIs	https://doi.org/10.1021/jf0306901
Publication status	Published - 2004 Mar 24

Keywords

1-Deoxynojirimycin
Evaporative light scattering detector
Morus alba
Mulberry leaves

ASJC Scopus subject areas

Chemistry(all)
Agricultural and Biological Sciences(all)

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10.1021/jf0306901

Cite this

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title = "Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection",

abstract = "A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.",

keywords = "1-Deoxynojirimycin, Evaporative light scattering detector, Morus alba, Mulberry leaves",

author = "Toshiyuki Kimura and Kiyotaka Nakagawa and Yuko Saito and Kenji Yamagishi and Masahiro Suzuki and Kohji Yamaki and Hiroshi Shinmoto and Teruo Miyazawa",

year = "2004",

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doi = "10.1021/jf0306901",

language = "English",

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T1 - Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection

AU - Kimura, Toshiyuki

AU - Nakagawa, Kiyotaka

AU - Saito, Yuko

AU - Yamagishi, Kenji

AU - Suzuki, Masahiro

AU - Yamaki, Kohji

AU - Shinmoto, Hiroshi

AU - Miyazawa, Teruo

PY - 2004/3/24

Y1 - 2004/3/24

N2 - A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.

AB - A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.

KW - 1-Deoxynojirimycin

KW - Evaporative light scattering detector

KW - Morus alba

KW - Mulberry leaves

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Determination of 1-Deoxynojirimycin in Mulberry Leaves Using Hydrophilic Interaction Chromatography with Evaporative Light Scattering Detection

Abstract

Keywords

ASJC Scopus subject areas

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