Detektion der DNA-Hybridisierung mittels Goldoberflächenmodifizierten lichtadressierbaren potentiometrischen Sensoren DNA-hybridization detection using light-addressable potentiometric sensor modified with gold layer

Chunsheng Wu, Thomas Bronder, Arshak Poghossian, Carl Frederik Werner, Michael J. Schöning

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Single-stranded deoxyribonucleic acid (ssDNA) probes modified with thiol group were immobilized on the patterned gold surface of a light-addressable potentiometric sensor (LAPS) via gold-thiol bond. The LAPS chip immobilized with ssDNA probe was used as a working electrode in a scanning LAPS measurement system. After the working electrode was incubated with a solution containing strands of complementary target ssDNA, the photocurrent-voltage curves of LAPS shift with increasing target ssDNA concentration. On the contrary, in a control experiment, practically no shift of photocurrent-voltage curve was observed if the LAPS chip was incubated with a solution containing mismatch ssDNA. This provides a convenient and label-free method for the detection of DNA hybridization and therefore, a promising approach for the development of novel LAPS-based DNA chips.

Original languageEnglish
Title of host publicationSensoren und Messsysteme 2014 - 17. ITG/GMA-Fachtagung
PublisherVDE Verlag GmbH
ISBN (Electronic)9783800736225
Publication statusPublished - 2013
Event17. ITG/GMA-Fachtagung Sensoren und Messsysteme 2014 - 17th ITG/GMA Conference on Sensors and Measuring Systems 2014 - Nurnberg, Germany
Duration: 2014 Jun 32014 Jun 4

Publication series

NameSensoren und Messsysteme 2014 - 17. ITG/GMA-Fachtagung

Conference

Conference17. ITG/GMA-Fachtagung Sensoren und Messsysteme 2014 - 17th ITG/GMA Conference on Sensors and Measuring Systems 2014
CountryGermany
CityNurnberg
Period14/6/314/6/4

ASJC Scopus subject areas

  • Information Systems
  • Control and Systems Engineering
  • Electrical and Electronic Engineering
  • Instrumentation
  • Atomic and Molecular Physics, and Optics

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