TY - JOUR
T1 - Detection of vitellogenin incorporation into zebrafish oocytes by FITC fluorescence
AU - Matsuda, Yohei
AU - Ito, Yoshie
AU - Hashimoto, Hisashi
AU - Yokoi, Hayato
AU - Suzuki, Tohru
N1 - Funding Information:
We thank Dr Akihiko Hara of Hokkaido University for advice on Vtg induction. This research was supported by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan to TS (22380104, 22658063).
PY - 2011/4/11
Y1 - 2011/4/11
N2 - Background: Large volumes of lymph can be collected from the eye-sacs of bubble-eye goldfish. We attempted to induce vitellogenin (Vtg) in the eye-sac lymph of bubble-eye goldfish and develop a method for visualizing Vtg incorporation by zebrafish oocytes using FITC-labeling.Methods: Estrogen efficiently induced Vtg in the eye-sac lymph of goldfish. After FITC-labeled Vtg was prepared, it was injected into mature female zebrafish.Results: Incorporation of FITC-labeled Vtg by zebrafish oocytes was detected in in vivo and in vitro experiments. The embryos obtained from zebrafish females injected with FITC-labeled Vtg emitted FITC fluorescence from the yolk sac and developed normally.Conclusion: This method for achieving Vtg incorporation by zebrafish oocytes could be useful in experiments related to the development and endocrinology of zebrafish oocytes.
AB - Background: Large volumes of lymph can be collected from the eye-sacs of bubble-eye goldfish. We attempted to induce vitellogenin (Vtg) in the eye-sac lymph of bubble-eye goldfish and develop a method for visualizing Vtg incorporation by zebrafish oocytes using FITC-labeling.Methods: Estrogen efficiently induced Vtg in the eye-sac lymph of goldfish. After FITC-labeled Vtg was prepared, it was injected into mature female zebrafish.Results: Incorporation of FITC-labeled Vtg by zebrafish oocytes was detected in in vivo and in vitro experiments. The embryos obtained from zebrafish females injected with FITC-labeled Vtg emitted FITC fluorescence from the yolk sac and developed normally.Conclusion: This method for achieving Vtg incorporation by zebrafish oocytes could be useful in experiments related to the development and endocrinology of zebrafish oocytes.
UR - http://www.scopus.com/inward/record.url?scp=79953782852&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79953782852&partnerID=8YFLogxK
U2 - 10.1186/1477-7827-9-45
DO - 10.1186/1477-7827-9-45
M3 - Article
C2 - 21481233
AN - SCOPUS:79953782852
VL - 9
JO - Reproductive Biology and Endocrinology
JF - Reproductive Biology and Endocrinology
SN - 1477-7827
M1 - 45
ER -