Detection of vitellogenin incorporation into zebrafish oocytes by FITC fluorescence

Yohei Matsuda; Yoshie Ito; Hisashi Hashimoto; Hayato Yokoi; Tohru Suzuki

doi:10.1186/1477-7827-9-45

Detection of vitellogenin incorporation into zebrafish oocytes by FITC fluorescence

Yohei Matsuda, Yoshie Ito, Hisashi Hashimoto, Hayato Yokoi, Tohru Suzuki

AGR - Bioscience and Biotechnology for Future Bioindustries

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Background: Large volumes of lymph can be collected from the eye-sacs of bubble-eye goldfish. We attempted to induce vitellogenin (Vtg) in the eye-sac lymph of bubble-eye goldfish and develop a method for visualizing Vtg incorporation by zebrafish oocytes using FITC-labeling.Methods: Estrogen efficiently induced Vtg in the eye-sac lymph of goldfish. After FITC-labeled Vtg was prepared, it was injected into mature female zebrafish.Results: Incorporation of FITC-labeled Vtg by zebrafish oocytes was detected in in vivo and in vitro experiments. The embryos obtained from zebrafish females injected with FITC-labeled Vtg emitted FITC fluorescence from the yolk sac and developed normally.Conclusion: This method for achieving Vtg incorporation by zebrafish oocytes could be useful in experiments related to the development and endocrinology of zebrafish oocytes.

Original language	English
Article number	45
Journal	Reproductive Biology and Endocrinology
Volume	9
DOIs	https://doi.org/10.1186/1477-7827-9-45
Publication status	Published - 2011 Apr 11

ASJC Scopus subject areas

Reproductive Medicine
Endocrinology
Obstetrics and Gynaecology
Developmental Biology

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title = "Detection of vitellogenin incorporation into zebrafish oocytes by FITC fluorescence",

abstract = "Background: Large volumes of lymph can be collected from the eye-sacs of bubble-eye goldfish. We attempted to induce vitellogenin (Vtg) in the eye-sac lymph of bubble-eye goldfish and develop a method for visualizing Vtg incorporation by zebrafish oocytes using FITC-labeling.Methods: Estrogen efficiently induced Vtg in the eye-sac lymph of goldfish. After FITC-labeled Vtg was prepared, it was injected into mature female zebrafish.Results: Incorporation of FITC-labeled Vtg by zebrafish oocytes was detected in in vivo and in vitro experiments. The embryos obtained from zebrafish females injected with FITC-labeled Vtg emitted FITC fluorescence from the yolk sac and developed normally.Conclusion: This method for achieving Vtg incorporation by zebrafish oocytes could be useful in experiments related to the development and endocrinology of zebrafish oocytes.",

author = "Yohei Matsuda and Yoshie Ito and Hisashi Hashimoto and Hayato Yokoi and Tohru Suzuki",

year = "2011",

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day = "11",

doi = "10.1186/1477-7827-9-45",

language = "English",

volume = "9",

journal = "Reproductive Biology and Endocrinology",

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TY - JOUR

T1 - Detection of vitellogenin incorporation into zebrafish oocytes by FITC fluorescence

AU - Matsuda, Yohei

AU - Ito, Yoshie

AU - Hashimoto, Hisashi

AU - Yokoi, Hayato

AU - Suzuki, Tohru

PY - 2011/4/11

Y1 - 2011/4/11

N2 - Background: Large volumes of lymph can be collected from the eye-sacs of bubble-eye goldfish. We attempted to induce vitellogenin (Vtg) in the eye-sac lymph of bubble-eye goldfish and develop a method for visualizing Vtg incorporation by zebrafish oocytes using FITC-labeling.Methods: Estrogen efficiently induced Vtg in the eye-sac lymph of goldfish. After FITC-labeled Vtg was prepared, it was injected into mature female zebrafish.Results: Incorporation of FITC-labeled Vtg by zebrafish oocytes was detected in in vivo and in vitro experiments. The embryos obtained from zebrafish females injected with FITC-labeled Vtg emitted FITC fluorescence from the yolk sac and developed normally.Conclusion: This method for achieving Vtg incorporation by zebrafish oocytes could be useful in experiments related to the development and endocrinology of zebrafish oocytes.

AB - Background: Large volumes of lymph can be collected from the eye-sacs of bubble-eye goldfish. We attempted to induce vitellogenin (Vtg) in the eye-sac lymph of bubble-eye goldfish and develop a method for visualizing Vtg incorporation by zebrafish oocytes using FITC-labeling.Methods: Estrogen efficiently induced Vtg in the eye-sac lymph of goldfish. After FITC-labeled Vtg was prepared, it was injected into mature female zebrafish.Results: Incorporation of FITC-labeled Vtg by zebrafish oocytes was detected in in vivo and in vitro experiments. The embryos obtained from zebrafish females injected with FITC-labeled Vtg emitted FITC fluorescence from the yolk sac and developed normally.Conclusion: This method for achieving Vtg incorporation by zebrafish oocytes could be useful in experiments related to the development and endocrinology of zebrafish oocytes.

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