Detection of Ultra Weak Chemiluminescence from Benzo(a)pyrene and 3-Methylcholanthrene

Kazumi Osada, Yuji Furukawa, Michio Komai, Shuichi Kimura

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

To study ultra weak chemiluminescence (CL) from poly-cyclic aromatic hydrocarbons, we examined the CL emission of benzo(a)pyrene (BP) and 3-methylcholanthrene (MC) in postmitochon-drial supernatant supplemented with NADPH. The integrated CL emission resulting from the metabolism of the BP and MC displayed saturation kinetics. In each case, the rate of CL emission correlated with the rate of metabolism; and by addition of trichloropropane oxide the CL intensity from BP and MC was decreased. This result suggests that epoxide hydrase is necessary for the CL emission. The CL from both compounds was similar in terms of emission spectrum, indicating that these compounds are metabolized by microsomal enzymes to form exci-plex species such as diol-dioxetane and diol-epoxide. Such species could cause both mutagenicity and CL emission.

Original languageEnglish
Pages (from-to)185-192
Number of pages8
JournalJournal of Clinical Biochemistry and Nutrition
Volume8
Issue number3
DOIs
Publication statusPublished - 1990 Jan

Keywords

  • benzo(a)pyrene
  • chemiluminescence
  • methylcholanthrene

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Clinical Biochemistry

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